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Regulation of vtg and VtgR in mud crab Scylla paramamosain by miR-34

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Abstract

Background

Vitellogenin (Vtg) is the precursor of major yolk protein and plays a crucial role in the maturation of oocytes and the production of eggs in oviparous animals. Vitellogenin receptor (VtgR) mediates the transport of Vtg explicitly to oocytes in the membrane. In a previous study, we found that miR-34 can regulate the expression of some eyestalk genes and affect reproduction in mud crab Scylla paramamosain, one of the most important economic crabs on the coasts of southern China.

Methods and results

In this study, firstly, we found that miR-34 can target at 3’-UTR of Vtg and VtgR genes by using bioinformatic tools and predicted miR-34 might depress the expression of Vtg and VtgR. Secondly, the relative luciferase activity of HEK293T cells co-transfected with miRNA mimic and pmir-RB-REPORTTM-Vtg/VtgR-3’UTR was significantly lower than those of cells co-transfected with mimic NC and pmir-RB-REPORTTM-Vtg/VtgR-3’UTR. Finally, in vivo experiments showed that agomiR-34 could repress the expression of Vtg and VtgR genes, while Antigomir-34 could promote the expression of these two genes.

Conclusions

These results confirm our hypothesis and previous published results that miR-34 may indirectly regulate ovarian development by binding to the 3’-UTR of Vtg and VtgR genes and inhibiting their expression.

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Funding

This study was supported by grants from the National Key R&D Program of China (2018YFD0900205), the National Natural Science Foundation of China (41676161, 31672681), Special Funds Provided by the Ministry of Science and Technology of the People’s Republic of China to Guide the Development of Science and Technology in Fujian Province (No.2020L3011), the Natural Science Foundation of Fujian Province (2019J01691).

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Authors and Affiliations

  1. Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, 361021, Xiamen, China

    Yinzhen Sheng, Jiaqian Liao, Yuting Li, Xiwei Jia, Xianyuan Zeng & Yilei Wang

  2. Fujian Engineering Research center of Aquatic Breeding and Healthy Aquaculture, 361021, Xiamen, China

    Yinzhen Sheng, Jiaqian Liao, Yuting Li, Xiwei Jia, Xianyuan Zeng & Yilei Wang

  3. College of Animal Science, Fujian Agriculture and Forestry University, 350002, Fuzhou, China

    Ziping Zhang

  4. Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, 350002, Fuzhou, China

    Ziping Zhang

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  1. Yinzhen Sheng
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  2. Jiaqian Liao
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  3. Ziping Zhang
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  4. Yuting Li
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Yinzhen Sheng, Jiaqian Liao, and Yuting Li. The first draft of the manuscript was written by Yinzhen Sheng and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Yilei Wang.

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All of the study design and animal experiments were conducted in accordance with the guidelines of Jimei University’s Animal Care and Use Committee (No.2021-4).

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Sheng, Y., Liao, J., Zhang, Z. et al. Regulation of vtg and VtgR in mud crab Scylla paramamosain by miR-34. Mol Biol Rep 49, 7367–7376 (2022). https://doi.org/10.1007/s11033-022-07530-x

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