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Comparative transcriptome analysis of leaf, stem, and root tissues of Semiliquidambar cathayensis reveals candidate genes involved in terpenoid biosynthesis

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Abstract

Background

Semiliquidambar cathayensis is a traditional medicinal plant and endemic species in China. Its roots, branches, leaves, bark, and nectar are known to have therapeutic effects against rheumatoid arthritis, lumbar muscle strain, and several other diseases. However, limited knowledge regarding the molecular properties of S. cathayensis highlights the need for further research in order to elucidate the underlying pathways governing the synthesis of its active ingredients and regulation of its accumulation processes.

Methods

We conducted transcriptome sequencing of the leaf, stem and root epidermises, and stem and root xylems of S. cathayensis with three biological replicates. Moreover, candidate genes involved in terpenoid biosynthesis, such as IDI, FPPS, DXR, SQS, GPPS, and HMGR were selected for quantitative real-time PCR analysis.

Results

We identified 88,582 unigenes. Among which, 36,144 unigenes were annotated to the nr protein database, 21,981 to the Gene Ontology database, 11,565 to the Clusters of Orthologous Groups database, 24,209 to the Pfam database, 21,685 to the SWISS-PROT database, and 12,753 to the Kyoto Encyclopedia of Genes and Genomes (KEGG), with 5072 unigenes common to all six databases. Of those annotated using the KEGG database, 187 unigenes were related to the terpenoid metabolism pathway, and expression analysis of the related genes indicated that the mevalonate and methylerythritol 4-phosphate pathways play different roles in terpenoid biosynthesis in different tissues of S. cathayensis.

Conclusions

These findings greatly expand gene resources of S. cathayensis and provide basic data for the study of the biosynthetic pathways and molecular mechanisms of terpenoids.

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Data availability

The data presented in this study are available upon request from the corresponding author. The RNA-seq Illumina paired-end reads of the transcriptome for this study have been submitted to NCBI BioProject PRJNA761214.

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Acknowledgements

The authors would like to thank Chunyan Pei for her assistance with our experiments.

Funding

This research was funded by Hunan Forestry Science and Technology Innovation Plan Project, Grant no XLK202106-2.

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Authors and Affiliations

  1. Hunan Botanical Garden, Changsha, 410116, China

    Xiaoming Tian, Lihong Yan, Liyuan Jiang, Guangfeng Xiang, Gaofei Li & Lu Zhu

  2. School of Computer Science and Engineering, Central South University, Changsha, 410083, China

    Jia Wu

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  1. Xiaoming Tian
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Correspondence to Xiaoming Tian or Jia Wu.

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Tian, X., Yan, L., Jiang, L. et al. Comparative transcriptome analysis of leaf, stem, and root tissues of Semiliquidambar cathayensis reveals candidate genes involved in terpenoid biosynthesis. Mol Biol Rep 49, 5585–5593 (2022). https://doi.org/10.1007/s11033-022-07492-0

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