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Development of novel polymorphic microsatellite markers for Picea brachytyla

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Abstract

Background

Picea brachytyla is a unique tree species in China. Due to being extensively exploited in the past, it is listed as Vulnerable in the IUCN Red List. It is mainly distributed across the Hengduan and Daba-Qinglin mountains and has been found in other areas including Sichuan Province and Qinghai Province, China. Microsatellites, or simple sequence repeats (SSRs), are widely used in correlational studies of genetic protection. Few markers have been developed for P. brachytyla because of the small number of trees and scholarly resources available for study.

Methods and Results

The genomic DNA of P. brachytyla was sequenced using the DNBSEQ platform, and unigenes were obtained after assembly and deredundancy. Of the 100 primer pairs screened, we isolated 10 useful microsatellite loci from P. brachytyla genes. The observed and expected heterozygosity values ranged from 0.173 (P24) to 0.788 (P79; mean 0.469) and 0.199 (P87) to 0.911 (P79; mean 0.700), respectively. Polymorphism-information content (PIC) ranged from 0.190 (P84) to 0.904 (P79; mean 0.666). Only P84 and P72 were in a Hardy-Weinberg equilibrium (P > 0.05) in the different P. brachytyla populations. All the levels of linkage disequilibrium (LD) were high for the 10 SSR loci indicating that there were no autocorrelations among the 10 SSR loci.

Conclusions

The novel polymorphic microsatellite markers showed high polymorphism for P. brachytyla. These polymorphic microsatellites can provide a basis for future conservation and genetic research on this rare plant species.

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Acknowledgements

We thank Dr. Le Wang, Dr. Biao Xiong, Dr. Fu-Yao Wei and Dr. Ying-Hua Wang for the helpful comments on the improvement of our manuscript. This research was supported by the Basic Research Project of Qinghai Province, China (No. 2019-ZJ-960Q) and the Thousand Talent Program of Qinghai Province.

Author information

Authors and Affiliations

  1. College of Agriculture and Animal Husbandry, Qinghai University, 810016, Xining, China

    Shuai-Peng Si & Chun-Jing Wang

  2. State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, 810016, Xining, China

    Ji-Zhong Wan & Chun-Hui Zhang

  3. Maixiu Forest Farm, 811401, Huangnan, China

    Yu-Lin Qian

Authors
  1. Shuai-Peng Si
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  2. Chun-Jing Wang
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  3. Ji-Zhong Wan
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  4. Yu-Lin Qian
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  5. Chun-Hui Zhang
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Contributions

Conceptualization and experimental design: Chun-Jing Wang, Ji-Zhong Wan; Formal analysis and investigation: Yu-Lin Qian, Chun-Hui Zhang, Ji-Zhong Wan; Project administration: Chun-Jing Wang; Writing—original draft: Shuai-Peng Si, Ji-Zhong Wan; Writing—review and editing: Chun-Jing Wang, Ji-Zhong Wan.

Corresponding authors

Correspondence to Chun-Jing Wang or Ji-Zhong Wan.

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Compliance with ethical standards

All field sampling work was allowed by the local government and all the experiments did not involve humans and animals. There was no issue on ethics in this study.

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The authors declare no conflicts of interest.

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Si, SP., Wang, CJ., Wan, JZ. et al. Development of novel polymorphic microsatellite markers for Picea brachytyla. Mol Biol Rep 49, 4095–4099 (2022). https://doi.org/10.1007/s11033-022-07346-9

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  • DOI: https://doi.org/10.1007/s11033-022-07346-9

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