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Study of the influence of NGF-β gene overexpression in human mesenchymal stem cells on the expression level of SOX1 and neural pathway genes

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Abstract

Background

Nerve growth factor (NGF) is a protein exhibiting an influence on the neural development and also, its’ impact on the stem cells remains a great potential treatment strategy. The influence of its overexpression on the neural pathway differentiation on Wharton’s Jelly derived MSC (WJ-MSC) has not been studied so far, but considering the fact that these cells are relatively easy to obtain, using them may indicate an innovative change in stem cell therapies. The aim of this study was to evaluate the effect of NGF overexpression in human mesenchymal stem cells (MSC) on SOX1 and genes related to the neural pathway.

Methods and results

The lentiviral transduction was performed in order to obtain the NGF overexpression, as well as RT-PCR to evaluate the expression level SOX1, SOX2, NES, NGF under influence of overexpressed NGF protein in WJ-MSC. During the study we have observed a decrease in SOX1 expression as the marker of neural stem cells. Other than that an increase of SOX2, NES and NGF was noticed, as they all are markers of early-neural as well as already differentiated neural cells. The results show a great potential of using those examined genes’ expression as a form of a new stem cell therapy.

Conclusions

The achieved overexpression of NGF in this study, led the modified MSC onto the neural pathway as well as caused a decrease of SOX1 expression and an increase of expression of genes related to neural differentiated cells.

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Acknowledgements

Authors would like to thank prof. Rafał Stojko MD, PhD for umbilical cords’ collection.

Funding

This work was supported by the Medical University of Silesia, grant number PCN-1-012/N/1/B.

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Authors and Affiliations

  1. Department of Medical Genetics, Medical University of Silesia, Jednosci 8 street, 41-200, Sosnowiec, Poland

    Julia Morys, Paulina Borkowska, Aleksandra Zielinska & Jan Kowalski

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  1. Julia Morys
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  2. Paulina Borkowska
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  3. Aleksandra Zielinska
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  4. Jan Kowalski
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Contributions

Study concept and design- PB; acquisition of data- JM, AZ.; analysis and interpretation of data- JM.; drafting the manuscript- JM; statistical analysis- PB, JM; technical or material support- JK; All authors have read and agreed to the published version of the manuscript.

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Correspondence to Julia Morys.

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Conflict of interest

The authors declare no relevant interests to disclose.

Ethics approval

The Bioethical Committee of the Medical University of Silesia in Katowice (Resolution No. KNW/022/KB/195/14) confirmed its acceptance for the entire technique of conducting this exploration.

Consent to participate

The volunteering mothers were informed of the procedure details. The study begun after receiving their full, written consent.

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Morys, J., Borkowska, P., Zielinska, A. et al. Study of the influence of NGF-β gene overexpression in human mesenchymal stem cells on the expression level of SOX1 and neural pathway genes. Mol Biol Rep 49, 4435–4441 (2022). https://doi.org/10.1007/s11033-022-07283-7

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  • DOI: https://doi.org/10.1007/s11033-022-07283-7

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