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Detection of nucleotide variants in FCGRT (Fc fragment of IgG, receptor, transporter, alpha) gene and their influence on colostral IgG concentration in Indian water buffalo (Bubalus bubalis)

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Abstract

Background

The neonatal Fc receptors (FcRn) mediate the transfer of immunoglobulin G (IgG) molecules from a dam’s circulation to the colostrum produced by it immediately after parturition. In ruminants, the calves born are agammaglobulinemic therefore, ingestion of colostrum with high concentration of IgG imparts passive immunity to the newborn. The FcRn molecule is a heterodimer, coded by FCGRT (Fc fragment of IgG Receptor Transporter neonatal) and B2M (Beta 2 microglobulin) genes. Present study attempted to identify single nucleotide polymorphisms (SNPs) in the FCGRT gene in 40 buffaloes of Murrah breed and evaluated the association of these nucleotide variations and haplotypes with IgG concentration in their colostrum.

Methods and results

Animals producing colostrum with high IgG and low IgG levels were identified by indirect ELISA and selected. SNPs were detected in the FCGRT gene sequence of selected animals by amplifying it in nine fragments covering all exons (with flanking introns) followed by PCR-single strand conformational polymorphism (PCR-SSCP). A total of nine SNPs were observed of which seven were present in flanking introns and two in exon 4 of the gene. The SNP A75G was non-synonymous and produced an amino acid change from isoleucine to valine. The exonic SNPs and corresponding haplotypes were found to be significantly (P < 0.01 and 0.05 respectively) associated with colostral IgG concentration based on Odds ratios at 95% confidence interval.

Conclusion

Polymorphism in FCGRT gene is found to be associated with IgG concentration in colostrum and identification of females with desirable variations may prevent failure of passive transfer in neonatal ruminants.

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Data availability

All relevant data and material for the study was provided by competent authorities at the Institute ICAR-IVRI.

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Consent for publication

Due consent was taken from all authors and competent authority for publication of the work.

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Acknowledgements

The authors extend their gratitude to The I/C Cattle and Buffalo Farm, ICAR-Indian Veterinary Research Institute, Izatnagar for providing intellectual inputs and physical resources.

Funding

This study was funded by Director, ICAR-Indian Veterinary Research Institute, Izatnagar.

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Authors and Affiliations

  1. Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP, 243122, India

    Swati Agrawal, Subodh Kumar, Rajni Chaudhary, Anuj Chauhan, Amit Kumar & B. Sivamani

  2. ICAR-North Temperate Research Station (ICAR-CSWRI), Garsa Distt., Kullu, Himachal Pradesh, India

    Rajni Chaudhary

  3. ICAR-Central Institute of Brackishwater Aquaculture, Chennai, Tamil Nadu, 600028, India

    B. Sivamani

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  1. Swati Agrawal
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  2. Subodh Kumar
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Contributions

SK: Conceptualization, Funding acquisition, Writing—review and editing. SA: Formal analysis and investigation, Writing—original draft preparation. RC: Methodology, Investigation. AC, AK: Conceptualization, Supervision. SB: Methodology.

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Correspondence to Subodh Kumar.

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Approval was taken from Institutional Animal Ethics Committee prior to blood collection.

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Agrawal, S., Kumar, S., Chaudhary, R. et al. Detection of nucleotide variants in FCGRT (Fc fragment of IgG, receptor, transporter, alpha) gene and their influence on colostral IgG concentration in Indian water buffalo (Bubalus bubalis). Mol Biol Rep 49, 3773–3781 (2022). https://doi.org/10.1007/s11033-022-07217-3

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  • DOI: https://doi.org/10.1007/s11033-022-07217-3

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