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The influence of gastric bypass surgery on the concentration of high mobility group box 1, nuclear factor erythroid 2–related factor 2 and the genes expression of high mobility group box 1, nuclear factor erythroid2–related factor 2, interleukin 6, and tumor necrosis factor-alpha in the peripheral blood mononuclear cells of patients with morbid obesity

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Abstract

Background and objectives

Obesity is known as a disease with a chronic low-grade state of inflammation and high levels of oxidative stress. Given the challenges and consequences caused by obesity, obesity therapy is an essential subject to address. For sustainable weight loss, gastric bypass surgery is the most successful and essential option.

Methods

This prospective cohort study was performed on 35 patients aged (18–54) with morbid obesity (BMI: 42.06 kg/m2). Volunteers blood was taken, and peripheral blood mononuclear cells (PBMCs) were isolated, high mobility group box 1(HMGB1), nuclear factor erythroid2–related factor 2(Nrf2), Interleukin 6(IL-6), tumor necrosis factor-alpha (TNF-α), and biochemical factors were determined one day before and 4 months after surgery.

Results

Four months following surgery, the BMI, hip and waist circumferences, and waist-to-hip ratio (WHR) all decreased significantly. The lipid profile and antioxidant power were dramatically enhanced after surgery. IL-6 and TNF-α expression in PBMC patients showed a significant decrease after surgery. HMGB1 and Nrf2 expression in PBMC of postoperative patients decreased compared to before surgery, and HMGB1, and Nrf2 protein levels also decreased after surgery.

Conclusion

Weight loss indicated the significant function of adipose tissue in the induction of oxidative stress and inflammatory factors. Gastric bypass reduced the inflammation conditions and improved the metabolic status and living situations in the patients with morbid obesity.

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Acknowledgements

This report was taken from a PhD thesis at Hamadan University of Medical Sciences, Iran. The research was funded by Vice-chancellor for Research and Technology, Hamadan University of Medical Sciences, Iran (No. 9811158686).

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Authors and Affiliations

  1. Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Masouod Khanaghaei, Nasrin Ziamajidi & Roghayeh Abbasalipourkabir

  2. Department of Epidemiology, School of Health, Hamadan University of Medical Sciences, Hamadan, Iran

    Jalal Poorolajal

  3. Department of Biochemistry and Nutrition, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

    Gholamreza Asadikaram

  4. Kerman Mehregan Bariatric Surgery Center, Kerman University of Medical Sciences, Kerman, Iran

    Payam Nikoyan & Mohammadhosein Bajian

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  1. Masouod Khanaghaei
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Correspondence to Roghayeh Abbasalipourkabir.

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Khanaghaei, M., Ziamajidi, N., Poorolajal, J. et al. The influence of gastric bypass surgery on the concentration of high mobility group box 1, nuclear factor erythroid 2–related factor 2 and the genes expression of high mobility group box 1, nuclear factor erythroid2–related factor 2, interleukin 6, and tumor necrosis factor-alpha in the peripheral blood mononuclear cells of patients with morbid obesity. Mol Biol Rep 49, 3745–3755 (2022). https://doi.org/10.1007/s11033-022-07214-6

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