Abstract
Background
Coronary artery disease (CAD) is considered to be one of the most pivotal causes of death in the world. Over the past two decades, significant changes occurred in the diagnosis, prognosis, and treatment of CAD, which has helped reduce mortality rates. microRNAs (miRs) are a class of more than 5000 non-encoding RNA molecules (21–25 nucleotides across the length) that regulate complex biological processes. Today, miRNAs are used to study cardiovascular diseases. In the present study, the expression of miR-146a،miR-27, miR-149, and miR-34a in plasma suffering from CAD and the control group were investigated.
Methods and results
The present research was performed on 30 men with CAD and 30 healthy men as controls. The expression levels of miR-146a, miR-27a, miR-149, and miR-34a in the plasma of patients with CAD and the control group were measured using real-time PCR. Also, the correlation between the expression of circulating miRs levels and biochemical LDL-C, HDL-C, BMI, and total cholesterol was evaluated. The expression of miR-27a in the plasma of the CAD group was higher than in the control group (p = 0.020). The expression of miR-146a was downregulated in CAD patients compared to normal subjects (p = 0. 026). However, the expression of miR-34a, miR-149 in the plasma of CAD patients was not significantly different with the control group. In addition to, a direct correlation was found between the expression of miR-146a and HDL-c, the expression of miR-27a and LDL-C and the expression of miR-34a and total cholesterol. Also, the negative correlation between expressions of miR-149 with BMI was reported.
Conclusion
The obtained results demonstrated that miRs were closely related to biochemical factors and it points out the fact that miRNAs can be applied as a potential strategy for diagnosis and treatment of CAD.
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Acknowledgements
The authors’ appreciate Yasuj the University of Medical Sciences for its financial support of this study.
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Dr. AM conceived and designed the evaluation and drafted the manuscript. Dr. EH participated in designing the evaluation, performed parts of the statistical analysis, and helped to draft the manuscript. SH re-evaluated the data, revised the manuscript, and collected the clinical data and Study supervision FS. Interpreted them and revised the manuscript BK. Re-analyzed the clinical, material support, and statistical data. All authors read and approved the final manuscript.
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Ethics approval this study was approved by Iran National Committee for Ethics in Biomedical Research (IR.YUMS.REC.1396.121).
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Hosseinpor, S., Khalvati, B., Safari, F. et al. The association of plasma levels of miR-146a, miR-27a, miR-34a, and miR-149 with coronary artery disease. Mol Biol Rep 49, 3559–3567 (2022). https://doi.org/10.1007/s11033-022-07196-5
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DOI: https://doi.org/10.1007/s11033-022-07196-5