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Effects of different energy diets on DNA methylation and mRNA expression in follicle stimulating hormone receptor gene promoter region of Duolang sheep during estrus

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Abstract

Background

The aim of this study was to study the relationship between the methylation level of the promoter region of follicle-stimulating hormone receptor (FSHR) gene and the mRNA expression of Duolang sheep fed different energy diets.

Methods

In this study, polyembryo estrus Duolang sheep under different energy levels were selected as the experimental subjects. Dietary nutrient level reference (NY/T 816-2004), medium energy level was 10.88 MJ/day, high and low energy groups were increased and decreased by 15% on the basis of medium energy level, respectively 12.51 MJ/day, 9.25 MJ/day. Through RNA and DNA extraction, qPCR, bisulfitegenomicse-quencing PCR (BSP), sequence matching and other analysis of ovarian tissue of Duolang sheep. The difference of DNA methylation level and mRNA expression of FSHR gene during estrus in Duolang sheep fed with different energy diets was detected.

Results

The results showed the expression level of FSHR in high energy group was significantly higher than that in low energy group (P < 0.01), the expression level of FSHR in high energy group was significantly higher than that in medium energy group (P < 0.05), and the expression level of FSHR in medium energy group was significantly higher than that in low energy group (P < 0.05). In the target fragment of the promoter region of the FSHR gene, the methylation rate was 25% in the high energy group, 50% in the normal group, and 75% in the low energy group.

Conclusions

This study revealed that different dietary energy levels had certain effects on the FSHR gene DNA methylation level and mRNA expression, and the expression level was negatively correlated with methylation level.

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Data availability

All data generated or analyzed during this study are included in this published article/as supplementary information files.

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Acknowledgements

Thanks to the above the author with the help of various aspects. Thanks for the project support of the Efficient Utilization of Tarim Sheep Germplasm Resources (Grant No. 2019CB010).

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Authors and Affiliations

  1. College of Animal Science, Tarim University, Alar, 843300, Xinjiang, China

    Bo Liu, Weikun Tao, Fei Huang, Xue Rui, Peng Niu & Qinghua Gao

  2. College of Life Sciences, Tarim University, Alar, 843300, Xinjiang, China

    Jie Wang, Di Fang & Qinghua Gao

  3. Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production & Construction Corps, Alar, 843300, Xinjiang, China

    Qinghua Gao

  4. Xinjiang Urumqi Animal Husbandry and Veterinary General Station, Urumqi, 830000, Xinjiang, China

    Hongjian Li

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  1. Bo Liu
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Contributions

BL, conceived, drafted and approved this research article. Conceptualization: QHG; Data curation: BL; formal analysis: BL; funding acquisition: QHG; investigation: BL, FH; methodology: BL, WKT, XR; project administration: QHG, HJL; resources: XR, PN; visualization: BL, XR; roles/writing—original draft: BL; writing—review and editing: JW, PN, FH, DF; all authors read and approved the manuscript.

Corresponding author

Correspondence to Qinghua Gao.

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The authors declare that there are no competing interests associated with the manuscript.

Ethical approval

All animal experiments were conducted according to the Regulations and Guidelines for Experimental Animals established by the Ministry of Science and Technology (Beijing, China, revised in 2004). The present study was approved by the Institutional Animal Care and Use Committee of Tarim University.

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Liu, B., Li, H., Tao, W. et al. Effects of different energy diets on DNA methylation and mRNA expression in follicle stimulating hormone receptor gene promoter region of Duolang sheep during estrus. Mol Biol Rep 49, 2565–2577 (2022). https://doi.org/10.1007/s11033-021-07058-6

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