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Assessment of Association between miR-146a Polymorphisms and Expression of miR-146a, TRAF-6, and IRAK-1 Genes in Patients with Brucellosis

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Abstract

Background

Brucellosis is a major zoonosis all over the world. MicroRNAs are significant gene expression regulators and could be involved during the infections and also genetic alterations in the miRNAs sequence can affect primary miRNAs and precursor miRNAs processing and thus alter miRNAs expression. Current research studied the impact of the miR-146a polymorphism on miR-146a, TRAF-6, and IRAK-1 genes expression in patients with brucellosis illness.

Methods and results

In this research, 25 patients with brucellosis and 25 healthy participants with determined genotypes for miR-SNP rs2910164 and miR-SNP rs57095329 were recruited. IRAK-1, TRAF-6, and miR-146a expressions in peripheral blood mononuclear cells (PBMCs) were specified by quantitative real- time PCR (qRT-PCR). Moreover, interleukin-1β (IL-1β) and tumor necrosis factor- alpha (TNF-α) serum levels were assessed by a sandwich enzyme-linked immunosorbent assay (ELISA) technique. There was no significant difference in the expression level of miR-146a, IRAK-1, and TRAF-6, among the patients with brucellosis and control group. TRAF-6 PBMCs expression levels in the distinctive genotypes of rs2910164 were significantly observed in patients (P = 0.048). No significant distinctions were found in miR-146a, IRAK-1, and TRAF-6 expression levels and among the rs57095329 different genotypes in brucellosis patients and controls. Meanwhile, no significant relationship was found between the rs2910164 and rs57095329 genotypes and the serum level of cytokines mentioned between the two groups. We did not find any association between expression of TRAF-6, miR-146a, and IRAK-1 in PBMCs, and cytokines serum levels with two single nucleotide polymorphisms (SNPs) in miR-146a.

Conclusions

To the best of writers’ knowledge, this research is the first one evaluating the probable link between the miR-146a rs2910164 and rs57095329 variant with miRNAs, relevant cytokine levels, and target genes in brucellosis.

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Data availability

The data analyzed are available from the corresponding author on reasonable request.

Code availability

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Acknowledgements

The authors express their gratitude to the Vice-chancellor of Research of Hamadan University of Medical Sciences, Hamadan, Iran for advocating this research. We also thanks Professor Ali Reza Soltanian for his cooperation in statistical analysis of the results.

Funding

This study was funded by the Hamadan University of medical sciences, Hamadan, IRAN, under Grant Number 9711307368.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Sima Kazemi & Mohammad Yousef Alikhani

  2. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Saeid Afshar & Massoud Saidijam

  3. Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran

    Fariba Keramat, Seyed Hamid Hashemi & Mohammad Yousef Alikhani

  4. Department of Biostatistics and Epidemiology, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran

    Manoochehr Karami

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  1. Sima Kazemi
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Contributions

The design and supervision of the study were conducted by MYA. SA and MS carried out data interpretation. SK collected the data and conducted the experiments. SHH and FK conducted clinical examinations. MK analyzed the numerical findings of the study. The manuscript has been read and approved by all the authors.

Corresponding author

Correspondence to Mohammad Yousef Alikhani.

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The authors state that there is no conflict of interest.

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The present study was ethically approved by the Hamadan University of Medical Sciences, Institutional Review Board (IR.UMSHA.REC.1397.857).

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Informed consent was obtained from all individual participants included in the study.

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Kazemi, S., Afshar, S., Keramat, F. et al. Assessment of Association between miR-146a Polymorphisms and Expression of miR-146a, TRAF-6, and IRAK-1 Genes in Patients with Brucellosis. Mol Biol Rep 49, 1995–2002 (2022). https://doi.org/10.1007/s11033-021-07014-4

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