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The link of ERCC2 rs13181 and ERCC4 rs2276466 polymorphisms with breast cancer in the Bangladeshi population

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Abstract

Background

Breast cancer (BC) is the most common disease in women and the leading cause of death from cancer globally. Epidemiological studies examined that nucleotide excision repair genes ERCC2 (rs13181) and ERCC4 (rs2276466) SNPs might increase cancer risk. Based on the previous investigation, this study was conducted to explore the correlation between these polymorphisms and BC susceptibility in Bangladeshi women.

Methods and results

Between January 2019 and January 2020, 140 blood samples were collected from female patients histologically diagnosed with BC, and 111 female controls were recruited from non-cancer subjects. Genotyping was performed applying the PCR–RFLP method, and all statistical analyzes were conducted using SPSS, version 25.0. Comparison of characteristics and clinicopathological features between ERCC2 rs13181 and ERCC4 rs2276466 carriers and non-carriers showed no significant link with BC. Analysis of ERCC2 rs13181 with the risk of BC showed that the GG genotype and G allele carriers showed a fourfold and 1.78-fold higher risk (OR 4.00, P = 0.001 and OR 1.78, P = 0.002) that are statistically significant. In addition, the patients with combined TG+GG genotype revealed a 2.09-fold increased chance (OR 2.09, P = 0.020) BC development. Analysis of recessive model (GG vs. TT+TG) also depicted 2.74-times significantly higher risk (OR 2.74, P = 0.002). On the other hand, ERCC4 rs2276466 polymorphism did not show any significant association with BC (P > 0.05).

Conclusions

Our findings show that ERCC2 rs13181 is linked to an elevated risk of BC. Our study also shows that ERCC4 rs2276466 polymorphism has no substantial risk of BC in the Bangladeshi population.

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Acknowledgements

The authors are grateful to Dhaka University’s authorities for granting permission to perform this research at the pharmacogenetics and pharmacokinetics laboratory. The authors are also grateful to the individuals who provided written consent to participate in this research and gave permission for their samples and clinical data to be used.

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Authors and Affiliations

  1. Department of Pharmacy, State University of Bangladesh, Dhaka, Bangladesh

    Shaid All Sahaba

  2. Department of Pharmaceutical Chemistry, University of Dhaka, Dhaka, Bangladesh

    Mohammad Abdur Rashid

  3. Department of Clinical Pharmacy and Pharmacology, University of Dhaka, Dhaka, Bangladesh

    Md. Saiful Islam, Noor Ahmed Nahid, Mohd Nazmul Hasan Apu, Taposhi Nahid Sultana, Nusrat Islam Chaity & Md. Mehedi Hasan

  4. Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali, 3814, Bangladesh

    Mohammad Safiqul Islam

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  1. Shaid All Sahaba
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Sahaba, S.A., Rashid, M.A., Islam, M.S. et al. The link of ERCC2 rs13181 and ERCC4 rs2276466 polymorphisms with breast cancer in the Bangladeshi population. Mol Biol Rep 49, 1847–1856 (2022). https://doi.org/10.1007/s11033-021-06994-7

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