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Galactose specific lectins from prostate tissue with different pathologies: biochemical and cellular studies

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Abstract

Background

Galectins—galactose-specific lectins are involved in various types of cell activities, including apoptosis, cell cycle regulation, inflammation and cell transformation. Galectins are implicated in prostate malignat transformation. It is not known yet if prostate glands with different grade of pathologies are expressing different galectins and if these galectins express different effects on the cell viability.

Methods

Cytosolic galactose-spesific lectin fractions from prostate tissue with different diagnosis were purified by affinity chromatography and analyzed by electrophoresis in polyacrylamide gel electrophoresis with sodium dodecyl sulphate. The lectin effects in a source-dependent maner were studied on cell viability on peripheral lymphocytes by MTT reduction method and on apoptosis by flow cytometry method.

Results

Affinity purified galactose-specific lectins fractions from normal and pathological tissue samples are characterized with different protein composition and they express different effects on cell viability and apoptosis.

Conclusion

The effects of cytosolic galactose-specific lectins depend on the source of lectin fraction (glandular tissue disease). We suppose that the released cytosolic galectins from prostatic high grade intraepithelial neoplasia and adenocarcinoma tissue could suppress the immune status of the host patients.

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Data availability

The authors confirm that the data supporting the findings of this study are available within the article.

Code availability

Not applicable.

Abbreviations

AAH:

Atypical adenomatous hyperplasia;

BPH:

Benign prostate hyperplasia

HGPIN:

High-grade intraepithelial neoplasia

LGPIN:

Low-grade intraepithelial neoplasia

PC:

Prostate adenocarcinoma

post-op:

Post operation

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Authors and Affiliations

  1. Department of Biology, Chair of Biochemistry, Faculty of Exact and Natural Sciences, Ivane Javakhishvili Tbilisi State University, Tbilisi, Georgia

    Elene Davitashvili, George Burjanadze, Marine Koshoridze & Nana Koshoridze

  2. Department of Biology Chair of Immunology and Microbiology, Faculty of Exact and Natural Sciences, Ivane Javakhishvili Tbilisi State University, Tbilisi, Georgia

    Tamar Tsertsvadze & Nunu Mitskevich

  3. A. Tsulukidze Urology National Center, Pathomorphology Laboratory 1st Clinical Hospital, Tbilisi, Georgia

    Omar Tsintsadze

  4. Clinic of SJC “Modern Medical Technologies”, Tbilisi, Georgia

    Guram Karazanashvili

  5. Institute of Chemical Biology, Ilia State University, Tbilisi, Georgia

    Revaz Solomonia

Authors
  1. Elene Davitashvili
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  2. George Burjanadze
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Contributions

The authors confirm contribution to the paper as follows: data collection and preparation of the figures—GB and MK. Investigation of lectins’ effect on cell cycle—TT and NM. Supplying the prostate post-operational tissue for further observations and tissue analyzes—GK (Surgeon) and OT. Statistical analyzes, co-writing and revision of the manuscript—NK and RS. Supervision, writing of the manuscript, revision and preparation for the submission, corresponding author—ED.

Corresponding author

Correspondence to Elene Davitashvili.

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Conflict of interest

The authors declare that there is no conflict of interest, including specific financial interests, relationships, or affiliations relevant to the subject matter or materials discussed in the manuscript.

Ethical approval

Approval for the use of the tissues was obtained from the local ethical committee at A. Tsulukidze Urology National Modern Center, Pathomorphology Laboratory at the 1st Clinical Hospital and Clinic of SJC "Modern Medical Technologies", and the patients' written consent. However, through dissemination process, we made sure that the patients' data protection system is firmly implemented and that no leakage of personal data or the results of the diagnosis, prognosis etc. into public domain takes place. This was insured through the implementation of modern guidelines of ethics and data protection as planned in this application. The study design was approved by the internal review board of the institution. The scientific research was carried out at Ivane Javakhishvili Tbilisi State University, Faculty of Exact and Natural Sciences, Department of Biology, in tight coordination of chairs of Biochemistry and Immunology/Microbiology. The ethical approval of the study as long as the guidelines followed to carry out the study was also obtained from National Center for disease control and public health (Institutional Review Board Letter 2021-069).

Consent to participate

Informed consent for using the surgical tissues for future scientific research was obtained from all patients.

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Not Applicable.

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Davitashvili, E., Burjanadze, G., Koshoridze, M. et al. Galactose specific lectins from prostate tissue with different pathologies: biochemical and cellular studies. Mol Biol Rep 49, 443–450 (2022). https://doi.org/10.1007/s11033-021-06894-w

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  • DOI: https://doi.org/10.1007/s11033-021-06894-w

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