Skip to main content
SpringerLink
Log in

Anshen-Buxin-Liuwei pill, a Mongolian medicinal formula could alleviate cardiomyocyte hypoxia/reoxygenation injury via mitochondrion pathway

  • Original Article
  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Background

Anshen Buxin Liuwei pill (ABLP) is a Mongolian medicinal formula that is composed of six medicinal materials: the Mongolian medicine Bos taurus domesticus Gmelin, Choerospondias axillaris (Roxb.) Burtt et Hill, Myristica fragrans Houtt., Eugenia caryophμllata Thunb., Aucklandia lappa Decne., and Liqui dambar formosana Hance. ABLP is considered to have a therapeutic effect on symptoms such as coronary heart disease, angina pectoris, arrhythmia, depression and irritability, palpitation, and shortness of breath.

Methods

H9c2 cardiomyocytes were used to construct a hypoxia/reoxygenation (HR) injury model. CCK-8 assay and Annexin V-FITC cell apoptosis assays were used for cell viability and cell apoptosis determination. The LDH, SOD, MDA, CAT, CK, GSH-Px, Na+-K+-ATPase, and Ca2+-ATPase activities in cells were determined to assess the protective effects of ABLP. The mRNA levels of Sirtuin3 (Sirt3) and Cytochrome C (Cytc) in H9c2 cells were determined by quantitative real-time PCR.

Results

The results indicate that HR-treated cells began to shrink from the spindle in an irregular shape with some floated in the medium. By increasing the therapeutic dose of ABLP (5, 25, and 50 μg/mL), the cells gradually reconverted in a concentration-dependent manner. The release of CK in HR-treated cells was significantly increased, indicating that ABLP exerts a protective effect in H9c2 cells against HR injury and can improve mitochondrial energy metabolism and mitochondrial function integrity. The present study scrutinized the cardioprotective effects of ABLP against HR-induced H9c2 cell injury through antioxidant and mitochondrial pathways.

Conclusions

ABLP could be a promising therapeutic drug for the treatment of myocardial ischemic cardiovascular disease. The results will provide reasonable information for the clinical use of ABLP.

Graphic abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Data availability

All data included in this study are available upon request by contact with the corresponding author.

References

  1. Thangarasu S, Jeganathan M, Krishna PM (2018) Sinapic acid protects heart against ischemia/reperfusion injury and H9c2 cardiomyoblast cells against oxidative stress. Biochem Biophys Res Comm 456(4):853–859. https://doi.org/10.1016/j.bbrc.2014.12.022

    Article  CAS  Google Scholar 

  2. Nie CQ, Ding X, Zheng M (2021) Hydrogen gas inhalation alleviates myocardial ischemia-reperfusion injury by the inhibition of oxidative stress and NLRP3-mediated pyroptosis in rats. Life Sci 272:119248. https://doi.org/10.1016/j.lfs.2021.119248

    Article  CAS  PubMed  Google Scholar 

  3. Tong HY, Zhang SQ, Murtaza G (2019) The present scenario, challenges, and future anticipation of traditional Mongolian medicine in China. World J Tradit Chin Med 5(4):187–192. https://doi.org/10.4103/wjtcm.wjtcm_34_19

    Article  Google Scholar 

  4. Lu JK, Hu YC, Wang LC (2018) Understanding the multitarget pharmacological mechanism of the traditional Mongolian Common herb pair GuangZao-RouDouKou acting on coronary heart disease based on a bioinformatics approach. Evid Based Complement Altern Med. https://doi.org/10.1155/2018/7956503

    Article  Google Scholar 

  5. Shen XN (2021) Clinical effect of Mongolian medicine Anshen-Buxin-Liuwei pill combined with metoprolol succinate sustained release tablets in the treatment of coronary heart disease and angina pectoris. Chin J Ethnomed Ethnopharm 27(01):13–14. https://doi.org/10.16041/j.cnki.cn15-1175.2021.01.008

    Article  Google Scholar 

  6. Okubo S, Ohta T, Fujita H (2020) Costunolide and dehydrocostuslactone from Saussurea lappa root inhibit autophagy in hepatocellular carcinoma cells. Nat Med 75(1):240–245. https://doi.org/10.1007/S11418-020-01462-1

    Article  Google Scholar 

  7. Lu T, Li YP, Hu J (2021) Dexpramipexole attenuates myocardial ischemia/reperfusion injury through upregulation of mitophagy. Eur J Pharmacol 899:173962–173962. https://doi.org/10.1016/J.EJPHAR.2021.173962

    Article  Google Scholar 

  8. Amin MZ, Narges M, Atkin SL (2018) The protective role of curcumin in myocardial ischemia-reperfusion injury. J Cell Physiol 234(1):214–222. https://doi.org/10.1002/jcp.26848

    Article  CAS  Google Scholar 

  9. Hausenloy DJ, Yellon DM (2013) Myocardial ischemia-reperfusion injury: a neglected therapeutic target. J Clin Invest 123(1):192–200. https://doi.org/10.1172/JCI62874

    Article  CAS  Google Scholar 

  10. Shoji S, Issei K, Masafumi K (2011) Pathophysiology of myocardial reperfusion injury: preconditioning, postconditioning, and translational aspects of protective measures. Am J Physiol Heart Circ Physiol 301(5):H1723–H1741. https://doi.org/10.1152/ajpheart.00553.2011

    Article  CAS  Google Scholar 

  11. Jiang HX, Wood KV, Morgan JA (2005) Metabolic engineering of the phenylpropanoid pathway in Saccharomyces cerevisiae. Appl Environ Microbiol 71(6):2962–2969. https://doi.org/10.1128/AEM.71.6.2962-2969.2005

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Katarzyna Z, Brandon F, Jordan M (2010) Naringenin, a citrus flavonoid, increases muscle cell glucose uptake via AMPK. Biochem Biophys Res Commun 398(2):178–183. https://doi.org/10.1016/j.bbrc.2010.06.048

    Article  CAS  Google Scholar 

  13. Kikuchi K, Tancharoen S, Takeshige N (2013) The efficacy of edaravone (Radicut), a free radical scavenger, for cardiovascular disease. Int J Mol Sci 14(7):13909–13930. https://doi.org/10.3390/ijms140713909

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Vishnupriya P, Padma V (2017) A review on the antioxidant and therapeutic potential of Bacopa monnieri. React Oxyg Species. https://doi.org/10.20455/ros.2017.817

    Article  Google Scholar 

  15. Malorni W, Testa U, Rainaldi G (1998) Oxidative stress leads to a rapid alteration of transferrin receptor intravesicular trafficking. Exp Cell Res 241(1):102–116. https://doi.org/10.1006/excr.1998.4020

    Article  CAS  PubMed  Google Scholar 

  16. Cao H, Xu H, Zhu GQ (2017) Isoquercetin ameliorated hypoxia/reoxygenation-induced H9c2 cardiomyocyte apoptosis via a mitochondrial-dependent pathway. Biomed Pharmacother 95:938–943. https://doi.org/10.1016/j.biopha.2017.08.128

    Article  CAS  PubMed  Google Scholar 

  17. Hallows WC, Smith BC, Lee S (2009) Ure(k)a! sirtuins regulate mitochondria. Cell 137(3):404–406. https://doi.org/10.1016/j.cell.2009.04.036

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Wu JJ, Yang YL, Gao YF (2020) Melatonin attenuates anoxia/reoxygenation injury by inhibiting excessive mitophagy through the MT2/SIRT3/FoxO3a signaling pathway in H9c2 cells. Drug Des Dev Ther 14:2047–2060. https://doi.org/10.2147/DDDT.S248628

    Article  CAS  Google Scholar 

  19. Yi XL, Guo WN, Shi Q (2019) SIRT3-dependent mitochondrial dynamics remodeling contributes to oxidative stress-induced melanocyte degeneration in vitiligo. Theranostics 9(6):1614–1633. https://doi.org/10.7150/thno.30398

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Gao JM, Liu S, Xu F (2018) Trilobatin protects against oxidative injury in neuronal PC12 cells through regulating mito-chondrial ROS homeostasis mediated by AMPK/Nrf2/Sirt3 signaling pathway. Front Mol Neurosci 11:267. https://doi.org/10.3389/fnmol.2018.00267

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Xiong SP, Sun HJ, Cao L (2019) Stimulation of Na+/K+-ATPase with an antibody against Its 4th extracellular region attenuates angiotensin II-induced H9c2 cardiomyocyte hypertrophy via an AMPK/SIRT3/PPAR-γ signaling pathway. Oxid Med Cell Longev 2019:4616034. https://doi.org/10.1155/2019/4616034

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Yin Y, Han W, Cao Y (2019) Association between activities of SOD, MDA and Na+-K+-ATPase in peripheral blood of patients with acute myocardial infarction and the complication of varying degrees of arrhythmia. Hellenic J Cardiol 60(6):366–371. https://doi.org/10.1016/j.hjc.2018.04.003

    Article  PubMed  Google Scholar 

  23. Chen Z, Jin ZS, Xia Y (2017) The protective effect of lipid emulsion in preventing bupivacaine-induced mitochondrial injury and apoptosis of H9c2 cardiomyocytes. Drug Deliv 24(1):430–436. https://doi.org/10.1080/10717544.2016.1261379

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Funding

This work was supported by grants from the National Key R&D Program of China (2018YFC1708206), Research on Identification and Quality Evaluation of Cross-border Traditional Medicine Resources between China and Pakistan (2020E01011), and the Fundamental Research Funds for the Central Universities, South-Central University for Nationalities (CZP20002).

Author information

Authors and Affiliations

  1. Department of Medicine and Pharmacy, College of Pharmacy, South-Central University for Nationalities, Wuhan, 430070, Hubei, China

    Yu-Jia Huang, Xian‑Ju Huang, Xin-Cai Xiao & Yue Dong

  2. School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, China

    Hai‑Ying Tong

  3. Department of Biotechnology, University of Okara, Okara, Pakistan

    Muhammad Sarfaraz Iqbal

Authors
  1. Yu-Jia Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hai‑Ying Tong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xian‑Ju Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xin-Cai Xiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yue Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Muhammad Sarfaraz Iqbal
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

H‑YT and X-JH contributed to the conception of the study. Y-JH experimented. Y-JH and X-JH contributed significantly to analysis and manuscript preparation. Y-JH and X-JH performed the data analyses and wrote the manuscript. X-CX, YD and MSI helped perform the analysis with constructive discussions.

Corresponding authors

Correspondence to Hai‑Ying Tong or Xian‑Ju Huang.

Ethics declarations

Conflict of interest

The authors declare that there are no conflict of interest.

Ethical approval

I testify on behalf of all co-authors that our article submitted to Molecular Biology Reports. All authors: (1) This material has not been published in whole or in part elsewhere; (2) The manuscript is not currently being considered for publication in another journal; (3) All authors have been personally and actively involved in substantive work leading to the manuscript and will hold themselves jointly and individually responsible for its content.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Huang, YJ., Tong, H., Huang, X. et al. Anshen-Buxin-Liuwei pill, a Mongolian medicinal formula could alleviate cardiomyocyte hypoxia/reoxygenation injury via mitochondrion pathway. Mol Biol Rep 49, 885–894 (2022). https://doi.org/10.1007/s11033-021-06867-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-021-06867-z

Keywords

Search

Navigation