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Circulating expression levels of CircHIPK3 and CDR1as circular-RNAs in type 2 diabetes patients

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Abstract

Background

Recent investigations suggested that deregulated levels of Circular RNAs (circRNAs) could be associated with type 2 diabetes mellitus (T2DM) pathogenesis. Accordingly, this study aimed to determine the expression levels of circulating CircHIPK3, CDR1as and their correlation with biochemical parameters in patients with T2DM, pre-diabetes and control subjects.

Methods and results

The expression of circRNAs in peripheral blood was determined using QRT-PCR in 70 patients with T2DM, 60 pre-diabetes and in 69 age and sex matched healthy controls. Moreover, bioinformatics tools were applied to explore and predict the potential interactions between circRNAs and other non-coding RNAs (ncRNAs). Our analysis revealed that the expression level of CircHIPK3 was significantly elevated in T2DM patients compared to healthy participants (P < 0.001) and pre-diabetes subjects (P = 0.018). In addition, ROC analysis suggested that at the cutoff value of 0.24 and the sensitivity and specificity of 50% and 88.4%, respectively, CircHIPK3 could distinguish between T2DM patients and control subjects. Furthermore, it was observed that the expression level of CDR1as is higher in pre-diabetic individuals than healthy individuals (P = 0.004). Finally, Spearman correlation analysis showed that there was a significant correlation between CircHIPK3 and CDR1as expression levels and clinical and anthropometrical parameters such as BMI, systolic and diastolic blood pressure, HbA1c and fasting blood glucose (P < 0.005).

Conclusions

The data of this study provided evidence that the expression levels of CircHIPK3, CDR1as increased in T2DM and pre-diabetes subjects, respectively.

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Funding

This work was financially supported by a Grant (960416) from the Deputy of Research, Yasuj University of Medical Sciences.

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Authors and Affiliations

  1. Department of Biochmistry, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran

    Farzaneh Rezaeinejad & Ali Mirzaei

  2. Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran

    Bahman Khalvati & Gholamabbas Sabz

  3. Department of Laboratory Sciences, Faculty of Paramedicine, Yasuj University of Medical Sciences, Yasuj, Iran

    Behnam Alipoor

Authors
  1. Farzaneh Rezaeinejad
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  2. Ali Mirzaei
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  3. Bahman Khalvati
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  4. Gholamabbas Sabz
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  5. Behnam Alipoor
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Contributions

BA planned the studies; BA and AM conducted analysis and interpretation of all experiments; FR and BK conducted all experiments. BA and GS wrote the manuscript.

Corresponding author

Correspondence to Behnam Alipoor.

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The authors declare no conflicts of interest.

Ethical approval

The study was approved by the Yasuj University of Medical Sciences Ethics Committee (IR.YUMS.REC.1397.153) and performed in compliance with the 1964 Helsinki declaration. Moreover, the informed consent was obtained from all subjects before enrolling in the study.

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Rezaeinejad, F., Mirzaei, A., Khalvati, B. et al. Circulating expression levels of CircHIPK3 and CDR1as circular-RNAs in type 2 diabetes patients. Mol Biol Rep 49, 131–138 (2022). https://doi.org/10.1007/s11033-021-06850-8

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  • DOI: https://doi.org/10.1007/s11033-021-06850-8

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