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Mycofabrication of AgONPs derived from Aspergillus terreus FC36AY1 and its potent antimicrobial, antioxidant, and anti-angiogenesis activities

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Abstract

Background

There is an emergency need for the natural therapeutic agents to treat arious life threatening diseases such as cardio- vascular disease, Rheumatoid arthritis and cancer. Among these diseases, cancer is found to be the second life threatening disease; in this view the present study focused to synthesize the silver oxide nanoparticles (AgONPs) from endophytic fungus.

Methods

The endophytic fungus was isolated from a medicinal tree Aegle marmelos (Vilva tree) and the potential strain was screened through antagonistic activity. The endophytic fungus was identified through microscopic (Lactophenol cotton blue staining and spore morphology in culture media) and Internal Transcribed Spacer (ITS) 1, ITS 4 and 18S rRNA amplification. The endophyte was cultured for the synthesis of AgONPs and the synthesized NPs were characterized through UV- Vis, FT- IR, EDX, XRD and SEM. The synthesized AgONPs were determined for antimicrobial, antioxidant and anti- angiogenic activity.

Results

About 35 pigmented endophytic fungi were isolated, screened for antagonistic activity against 12 pathogens and antioxidant activity through DPPH radical scavenging assay; among the isolates, FC36AY1 explored the highest activity and the strain FC36AY1 was identified as Aspergillus terreus. The AgONPs were synthesized from the strain FC36AY1 and characterized for its confirmation, functional groups, nanostructures with unit cell dimensions, size and shape, presence of elements through UV–Vis spectrophotometry, FT-IR, XRD, SEM with EDX analysis. The myco-generated AgONPs manifested their antimicrobial and antioxidant properties with maximum activity at minimum concentration. Moreover, the inhibition of angiogenesis by the AgONPs in Hen’s Egg Test on the Chorio-Allantoic Membrane analysis were tested on the eggs of Chittagong breed evinced at significant bioactivity least concentration at 0.1 µg/mL.

Conclusions

Thus, the results of this study revealed that the fungal mediated AgONPs can be exploited as potential in biomedical applications.

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Acknowledgements

The authors were grateful to their institutional authorities for their support and also thankful to the Department of Nanoscience, Bharathiar University, Coimbatore, India for facilitating the instruments.

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Author notes

  1. Manon Mani Vellingiri and John Kennedy Mithu Ashwin are Equally contributed as the first author.

Authors and Affiliations

  1. Department of Biotechnology, Rathnavel Subramaniam College of Arts and Science, Coimbatore, Tamil Nadu, 641 402, India

    Manon Mani Vellingiri & Arockiam Jeyasundar Parimala Gnana Soundari

  2. Pasteur Institute of India, Coonoor, Tamil Nadu, 643103, India

    John Kennedy Mithu Ashwin

  3. Department of Microbial Biotechnology, Bharathiar University, Coimbatore, Tamil Nadu, 641 046, India

    Manon Mani Vellingiri, Swamiappan Sathiskumar & Ulaganathan Priyadharshini

  4. Department of Advanced Studies, The Gandhigram Rural Institute (Deemed to be University), Dindigul, India

    Arockiam Jeyasundar Parimala Gnana Soundari

  5. Department of Biotechnology, Dr. N.G.P. Arts and Science College (Autonomous and Affiliated to Bharathiar University), Coimbatore, Tamil Nadu, 641048, India

    Deepak Paramasivam

  6. Department of Animal Science, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, People’s Republic of China

    Wen-Chao Liu

  7. Department of Food Science and Biotechnology, College of Life Science, Sejong University, Seoul, 05006, South Korea

    Balamuralikrishnan Balasubramanian

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  1. Manon Mani Vellingiri
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Contributions

VMM: data curation, methodology, writing—original draft; JKMA: methodology, formal analysis; AJPGS: data curation, methodology, formal analysis; SS: methodology; UP: data curation, methodology; PD: formal analysis, resources; WL: data validation and interpretation, writing—review and editing; BB: conceptualization, validation, visualization, writing—review and editing.

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Correspondence to Wen-Chao Liu or Balamuralikrishnan Balasubramanian.

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Vellingiri, M.M., Ashwin, J.K.M., Soundari, A.J.P.G. et al. Mycofabrication of AgONPs derived from Aspergillus terreus FC36AY1 and its potent antimicrobial, antioxidant, and anti-angiogenesis activities. Mol Biol Rep 48, 7933–7946 (2021). https://doi.org/10.1007/s11033-021-06824-w

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