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Neuroprotective effect of bromelain in 6-hydroxydopamine induced in vitro model of Parkinson’s disease

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Abstract

Background

This study was designed to investigate the neuroprotective effects of bromelain, which is known to have anti-oxidant and anti-inflammatory properties, against the neurotoxicity (induced by 6-OHDA) in SH-SY5Y cells.

Methods and results

To establish Parkinson’s Disease (PD) model in cell culture conditions, SH-SY5Y cells were exposed to 200 µM 6-OHDA for 1 day. Prior to 6-OHDA treatment, SH-SY5Y cells had been pre-treated with bromelain (25 µg/mL, 50 µg/mL, 75 µg/mL and 100 µg/mL). After 1 day, cell viability was determined with the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) and lactate dehydrogenase (LDH) assays. Oxidative stress was assessed with total antioxidant capacity (TAC), total oxidant status (TOS), glutathione reductase (GR) and malondialdehyde (MDA) analyses. The effect of the bromelain in SH-SY5Ycells was also examined by 4′,6-diamidino-2-phenylindole (DAPI) staining. We found that 6-OHDA increased LDH leakage, and cellular apoptosis in SH-SY5Y cells. 6-OHDA aggravated oxidative stress by increasing TOS, MDA and GR and eventually promoted apoptosis in SH-SY5Y cells, while pretreatment with bromelain attenuated these toxic effects of 6-OHDA.

Conclusions

These findings indicated that bromelain, with its neuroprotective features can be useful for neuroprotection in PD.

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Data availability

Data and materials are available from the authors upon request.

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Funding

The authors did not receive any financial support.

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Authors and Affiliations

  1. Department of Pharmacology, Faculty of Pharmacy, Ataturk University, Yakutiye, 25100, Erzurum, Turkey

    Irmak Ferah Okkay

  2. Department of Medical Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey

    Ufuk Okkay, Aysegul Yilmaz, Fatma Yesilyurt & Ahmet Hacimuftuoglu

  3. Department of Physiology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan, Turkey

    Betul Cicek

  4. Department of Pathology, Faculty of Veterinary Medicine, Erciyes University, Kayseri, Turkey

    Ali Sefa Mendil

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Contributions

IFO and UO performed the experiments and wrote the manuscript; BC and ASM contributed significantly to the experiments and data analysis; AH contributed to the conception of the study; others assisted with the experiments.

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Correspondence to Irmak Ferah Okkay.

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Ferah Okkay, I., Okkay, U., Cicek, B. et al. Neuroprotective effect of bromelain in 6-hydroxydopamine induced in vitro model of Parkinson’s disease. Mol Biol Rep 48, 7711–7717 (2021). https://doi.org/10.1007/s11033-021-06779-y

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