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Development of the new microsatellite markers of Lucilia sericata (Diptera: Calliphoridae) from Korea

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Abstract

Background

Lucilia sericata is a medical and veterinary important insect species because its larvae feed on tissues of vertebrates including humans. Very few microsatellite makers have been reported from the species to illuminate its genetic variability and population genetic structure.

Methods and Results

In this study, L. sericata samples were collected from four different localities in Korea to develop the microsatellite markers to provide basic information on the genetic variability and population genetic structure in Korea of this species. In total, ten new microsatellite markers were sequenced and analyzed. Genetic diversity was performed using these microsatellite markers. The observed heterozygosity varied from 0.205 to 0.824, with an average of 0.546. The expected heterozygosity ranged from 0.579 to 0.886, with an average of 0.804. PIC value varied from 0.553 to 0.876.

Conclusions

The markers developed in the present study are expected as informative for estimating genetic diversity of L. sericata.

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Acknowledgements

Sincere appreciation is expressed to Mr. Jung-Il Kim, Mr. Tae-June Choi and Mr. Jong-Won Baek (Department of Biotechnology, Sangmyung University) for supporting sample collection for this study.

Funding

This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MSIP) [No. NRF-2016M3A9E1915578].

Author information

Authors and Affiliations

  1. Department of Biotechnology, Sangmyung University, Seoul, Korea

    Hyung-Eun An, Dinh Thinh Do, Duri Lee & Chang-Bae Kim

  2. 3Billion Inc., Seoul, Korea

    Mustafa Zafer Karagozlu

  3. Department of Legal Medicine, Korea University College of Medicine, Seoul, Korea

    Seong Hwan Park & Sang-Eon Shin

Authors
  1. Hyung-Eun An
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  2. Dinh Thinh Do
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  3. Duri Lee
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  4. Mustafa Zafer Karagozlu
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  5. Seong Hwan Park
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  6. Sang-Eon Shin
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  7. Chang-Bae Kim
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Contributions

CBK, HEA, MZK and SHP conceptualized the study, HEA, DL and SES performed the experiments, HEA, DTD and CBK wrote the final draft of manuscript, CBK revised the final draft of manuscript.

Corresponding author

Correspondence to Chang-Bae Kim.

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The authors declare no conflict of interest.

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An, HE., Do, D.T., Lee, D. et al. Development of the new microsatellite markers of Lucilia sericata (Diptera: Calliphoridae) from Korea. Mol Biol Rep 48, 8245–8248 (2021). https://doi.org/10.1007/s11033-021-06750-x

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  • DOI: https://doi.org/10.1007/s11033-021-06750-x

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