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Expression of cell proliferation regulatory factors bricd5, tnfrsf21, cdk1 correlates with expression of clock gene cry1 in testes of Hu rams during puberty

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Abstract

Background

Cryptochrome 1 (cry1), the core regulator of the circadian clock, is essential for ontogeny and mammalian reproduction. Unlike in other tissues, the cry1 gene have noncircadian functions in spermatogenesis, which implies the unique role of cry1 gene in the development of testis. The role of cry1 during the puberty has not been described yet. This study aimed to explore the relationship between cry1 expression and spermatogenic cell numbers.

Methods and results

We analyzed testicular tissues from Hu sheep aged 0–180 days by hematoxylin and eosin staining, measured cry1 and cell proliferation regulatory factors (bricd5, tnfrsf21, cdk1) expression by quantitative real-time PCR and characterized the transcription factor in the 5′ flanking region of cry1 gene. The data revealed that the number of spermatocytes and early spermatocytes increased rapidly from 90 to 120 dpp (day postpartum). Correspondingly, there was a marked variation in the cry1 and cell proliferation related genes (bricd5, tnfrsf21, cdk1) mRNA expression in the testes from the age of 90 days to 180 days (p < 0.05). We also identified some transcription factors (tcfl5) related to cell proliferation.

Conclusions

There is a significant causal relationship between the transcription level of cry1 gene in Hu sheep testes and the number of spermatogenic cells. It is speculated that cry1 gene may regulate the proliferation of spermatogenic cells by regulating the expression of cell proliferation related genes such as bricd5, tnfrsf21 and cdk1.

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Acknowledgements

We are thankful to Chenhui Liu, Dandan Du and all members of the Jiang laboratory who provided expertise that greatly assisted the research.

Funding

Supported by China Agriculture Research System of MOF and MARA (CARS-38). The funding bodies played no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.

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Author notes

  1. Yongjie Huang and Xunping Jiang have contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Small Ruminant Genetics, Breeding and Reproduction, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Yongjie Huang, Xunping Jiang, Yinan Yan, Guiqiong Liu & Chenhui Liu

  2. Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, Wuhan, 430070, People’s Republic of China

    Yongjie Huang, Xunping Jiang, Yinan Yan, Guiqiong Liu & Chenhui Liu

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  1. Yongjie Huang
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  2. Xunping Jiang
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  4. Guiqiong Liu
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  5. Chenhui Liu
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Contributions

Conceptualization, XJ, and GL; Methodology, YH, YY and CL; Investigation, YH; Supervision, XJ; Writing—Original Draft, YH and XJ; Writing—Review & Editing, all authors.

Corresponding author

Correspondence to Guiqiong Liu.

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The authors declare that they have no conflict of interest.

Ethical approval

The main research project researcher of this work was approved for animal ethics by the Scientific Ethic Committee of Laboratory Animal Centre, Huazhong Agricultural University (HZAUGO-2018–006, 1 March 2018), China. All applicable guidelines for the care and use of animals were followed by the authors.

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Huang, Y., Jiang, X., Yan, Y. et al. Expression of cell proliferation regulatory factors bricd5, tnfrsf21, cdk1 correlates with expression of clock gene cry1 in testes of Hu rams during puberty. Mol Biol Rep 48, 7379–7385 (2021). https://doi.org/10.1007/s11033-021-06747-6

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