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PVT1 and ZFAS1 lncRNAs expressions and their biomarker value in gastric cancer tissue sampling among Iranian population

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Abstract

Background

lncRNAs are modulatory factors with critical function in the tumorigenesis pathways, introducing them as promising therapeutic and diagnostic biomarkers for different cancers. This study is thus aimed to evaluate the differences in PVT1 and ZFAS1 gene expression in tumorous tissues as compared with adjacent healthy non-tumorous biopsies of gastric cancer cases.

Methods

One hundred two pairs of tumorous and adjacent non-tumorous biopsies of GC cases were sampled. RNA isolation and cDNA production were carried out. The qRT-PCR was performed to evaluate the expression of PVT1 and ZFAS1 genes. Moreover, the associations between PVT1 or ZFAS1 and clinicopathological characteristics as well as the biomarker roles of the lncRNAs were assessed.

Results

The PVT1 and ZFAS1 expressions showed a significant increase and decrease in GC samples as compared with non-cancerous tissues, respectively. PVT1 expression was significantly associated with and lymph-node involvement (p = 0.0007). Moreover, ZFAS1 expression demonstrated a significant association with lymph-node involvement (p = 0.0005), and tumor size >5 cm (p = 0.003). The findings of the ROC curve revealed that PVT1 and ZFAS1 may act as a possible biomarker with AUC of 0.71 and 0.79, specificity of 78.43% and 79.41%, and sensitivity of 55.88% and 64.71%.

Conclusions

Regarding upregulation of PVT1 and downregulation of ZFAS1 in human GC samples, these genes may respectively act as oncogenic and tumor-suppressive factors in GC cases. Furthermore, PVT1 and ZFAS1 can be considered as possible biomarkers for the detection and treatment of GC cases.

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Data availability

The data are available if requested by the Editor or reviewers.

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Acknowledgements

This work was a part of the project entitled “The assessment of non-coding RNAs function in human malignancies” (Grant Number: 3522) performed at the University of Tabriz. The authors appreciate the participation of patients in this study.

Funding

This project has been financially supported by the University of Tabriz (Grant Code: 3522).

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Authors and Affiliations

  1. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

    Narges Dastmalchi, Samaneh Tayefeh-Gholami, Ali Rajabi & Reza Safaralizadeh

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  1. Narges Dastmalchi
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  2. Samaneh Tayefeh-Gholami
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  3. Ali Rajabi
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  4. Reza Safaralizadeh
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Correspondence to Reza Safaralizadeh.

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The research was approved by the ethical committee of the Medical University of Tabriz with the code of IR.TABRIZU.REC.1398.015.

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Dastmalchi, N., Tayefeh-Gholami, S., Rajabi, A. et al. PVT1 and ZFAS1 lncRNAs expressions and their biomarker value in gastric cancer tissue sampling among Iranian population. Mol Biol Rep 48, 7171–7177 (2021). https://doi.org/10.1007/s11033-021-06709-y

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  • DOI: https://doi.org/10.1007/s11033-021-06709-y

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