Abstract
Background
The aim of this study was to elucidate the features of the expression of matrix metalloproteinases inducer—EMMPRIN (EMN) and matrix metalloproteinase 1 (MMP-1) in cell lines and in clinical samples of cervical squamous cell carcinoma (SCC).
Material and methods
The study was carried out using RT-PCR, densitometry and immunohistochemical studies (IHC) on commercial cell lines Siha, Caski, transformed with HPV16; HeLa, and C33A transformed with HPV18, line C33A without HPV, and in clinical samples of SCC and morphologically normal tissue adjacent to the tumor.
Results
The data obtained indicate that the expression of mRNA EMN and MMP-1 occurs in all cell lines at different levels. HPV type and number of genes copies had no effect on expression degree both EMN and MMP-1. Gene expression of EMN and MMP-1 has been investigated in tumor and normal tissues. MMP-1 expression in tumor tissue in SCC, as a rule, has been significantly increased (2–6 times) compared to normal tissue. It was found in 90% of tumor samples. It is known, that MMP-1 promotes the development of invasive and metastatic processes. EMN expression was lower in the tumor tissue than in normal tissue in most cases. An increase in EMN expression was noted only in some cases of SCC.
Conclusion
The data obtained indicate that MMP-1 can serve as a marker of the invasive potential of SCC. EMN, apparently, is not a major factor responsible for MMP-1 expression in SCC. Data are important for understanding the process of tumor development and may have prognostic value for the patient.
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Data availability
All the data on the basis of which the conclusions in this article are made are presented in this manuscript.
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The work was done in the framework of the Russian Federation fundamental research program for the long-term period for 2021-2030
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Russian Federation fundamental research program.
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NIS—the concept of the research, data analysis, writing articles; OST—isolation RNA from cells and tissues, RT-PCR, separation of products in agarose gel; EVK—preparation of experimental samples from postoperative tissues, carrying out an immunohistochemical reaction; TAG—cultivation of cell cultures. All authors read and approved the manuscript.
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Samples of squamous cell carcinomas (SCC) of the cervix were obtained from tumor tissue bank of N.N. Blokhin National Medical Research Center of Oncology under the approval of the Ethical Board of the Center. Informed consent was obtained from all patients.
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Solovyeva, N.I., Timoshenko, O.S., Kugaevskaya, E.V. et al. Interstitial collagenase MMP-1 and EMMPRIN in cell lines and in clinical specimens of cervical squamous cell carcinoma. Mol Biol Rep 48, 6879–6886 (2021). https://doi.org/10.1007/s11033-021-06689-z
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DOI: https://doi.org/10.1007/s11033-021-06689-z