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New microsatellite loci for estimating genetic diversity and structure in Octopus hubbsorum from Nayarit, México

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Abstract

Background

Octopus hubbsorum Berry, 1953 is the most important species for commercial fishing in the Mexican Pacific. However, there is a lack of information regarding population structure that could have important management implications. We tested 44 microsatellite loci in O. hubbsorum by cross-amplification from O. bimaculatus.

Methods and results

Genetic diversity and structure was tested over 30 octopus sampled from Santa Cruz de Miramar (Nayarit, México). A total of 11 loci were successfully amplified. All loci were polymorphic with the number of effective alleles ranging from 2.13 to 23.14, while three loci significantly deviated from Hardy-Weinberg equilibrium. No significant LD was observed between pairs of loci (P ≥ 0.05). The application of the new markers in a O. hubbsorum population from Santa Cruz de Miramar Nayarit, México, did not showed Wahlund or isolate breaking effects due to the mixing of distinct populations.

Conclusions

The loci were useful to estimate levels of pairwise relatedness and to discard the presence of recent demographic bottlenecks in the population. We consider that eight microsatellites are adequate from the 11 amplified loci

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Acknowledgements

We thank Consejo Nacional de Ciencia y Tecnología (CONACyT) and Posgrado en Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Nayarit and Secretaria de Investigación y Posgrado-Instituto Politécnico Nacional for the support provided to develop this project. JGA, AMV and FJGR are fellows of Sistema Nacional de Investigadores-Consejo Nacional de Ciencia y Tecnología (SNI-CONACyT). FJGR is fellow of Comisión de Operación y Fomento de Actividades Académicas-Instituto Politécnico Nacional (COFAA-IPN) and Estímulos al Desempeño de los Investigadores-Instituto Politécnico Nacional (EDI-IPN).

Funding

This study was funded by Fondo Ciencia Básica CONACyT (Grant No. 253315) and Secretaría de Investigación y Posgrado-Instituto Politécnico Nacional (SIP-IPN, SIP-20180539).

Author information

Authors and Affiliations

  1. Programa de Doctorado en Ciencias Biológico Agropecuarias, Universidad Autónoma de Nayarit, Xalisco, Nayarit, México

    José de Jesús Dueñas-Romero

  2. Instituto Politécnico Nacional-Centro Interdisciplinario de Ciencias Marinas (CICIMAR-IPN), Ave. IPN S/N, Col. Playa Palo de Santa Rita, CP 23096, La Paz, B.C.S., Mexico

    José Francisco Domínguez-Contreras & Francisco Javier García-Rodríguez

  3. Escuela Nacional de Ingeniería Pesquera, Universidad Autónoma de Nayarit (ENIP-UAN), Apartado Postal 10, C.P. 63740, San Blas, Nayarit, Mexico

    Jasmín Granados-Amores

  4. Conservation Genetics Laboratory & Desert Laboratory on Tumamoc Hill, The University of Arizona, Tucson, AZ, USA

    Adrián Munguía-Vega

Authors
  1. José de Jesús Dueñas-Romero
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  2. José Francisco Domínguez-Contreras
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  3. Jasmín Granados-Amores
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  4. Adrián Munguía-Vega
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  5. Francisco Javier García-Rodríguez
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Contributions

All authors of the paper have directly participated in the planning, execution, and analysis of this study.

Corresponding author

Correspondence to Francisco Javier García-Rodríguez.

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All authors have read and approved the final version submitted.

Ethical approval

Octopus hubbsorum is not an endangered or a protected species in the area sampled. Sampling activities were not performed at locations where specific permission is required.

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de Jesús Dueñas-Romero, J., Domínguez-Contreras, J.F., Granados-Amores, J. et al. New microsatellite loci for estimating genetic diversity and structure in Octopus hubbsorum from Nayarit, México. Mol Biol Rep 48, 7007–7012 (2021). https://doi.org/10.1007/s11033-021-06601-9

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