Abstract
Background
Crossostephium chinense is a traditional Chinese medicinal herb and it is often cultivated as an ornamental plant. Previous studies on this species mainly focused on its chemical composition and it was rarely represented in genetic studies, and thus genomic resources remain scarce.
Methods and results
Both chloroplast and nuclear polymorphic microsatellites of C. chinense were screened from genome skimming data of two individuals. 64 and 63 cpSSR markers were identified from two chloroplast genomes of C. chinense. A total of 133 polymorphic nSSRs were developed. Ten nSSRs were randomly selected to test their transferability across 35 individuals from three populations of C. chinense, and 20 individuals each of Artemisia stolonifera and A. argyi. Cross-amplifications were successfully done for C. chinense and were partially amplified for both Artemisia species. The number of alleles varied from two to nine. The observed heterozygosity and expected heterozygosity per locus ranged from 0.000 to 0.286 and from 0.029 to 0.755, respectively.
Conclusions
In this study, we developed polymorphic cpSSRs and nSSRs markers for C. chinense based on genome skimming sequencing. These genomic resources will be valuable for population genetics and conservation studies in C. chinense and Artemisia.
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Acknowledgements
This research was supported by the National Natural Science Foundation of China (Grant Nos. 31900188, 31970225), Natural Science Foundation of Zhejiang Province (Grant No. LY19C030007). The material was supported by National Wild Plant Germplasm Resource Center for Shanghai Chenshan Botanical Garden (Grant No. ZWGX1902).
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PL designed the study. PL, SS, YF and BJG collected the samples. LXL conducted the laboratory experiments. LXL, SLL, and KK conducted bioinformatic and statistical analyses. All authors drafted and revised the manuscript.
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Table S1
Locality and voucher information for populations of Crossostephium chinense, Artemisia stolonifera, and A. argyi in this study. Supplementary file1 (DOCX 15 kb)
Table S2
The detail information of polymorphic nSSRs identified for Crossostephium chinense and Primer pairs designed for each polymorphic nSSRs. Supplementary file2 (XLSX 61 kb)
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Liu, L., Low, S.L., Sakaguchi, S. et al. Development of nuclear and chloroplast polymorphic microsatellites for Crossostephium chinense (Asteraceae). Mol Biol Rep 48, 6259–6267 (2021). https://doi.org/10.1007/s11033-021-06590-9
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DOI: https://doi.org/10.1007/s11033-021-06590-9