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TCF7L2 gene polymorphism as a risk for type 2 diabetes mellitus and diabetic microvascular complications

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Abstract

Background

Type 2 Diabetes Mellitus (T2DM) is a chronic metabolic condition with various genetics and environmental influences that affects the capacity of the body to produce or use insulin resulting in hyperglycemia, which may lead to variable complications. It is one of the world’s rising health problems. There is emerging evidence that some genetic polymorphisms can impact the risk of evolving T2DM. We try to determine the relationship of (rs7903146) variant of the Transcription factor 7-like 2 (TCF7L2) gene with T2DM and its microvascular complications.

Methods and results

This case–control study included 180 subjects: 60 diabetic patients without complications, 60 diabetic patients with microvascular complications and 60 matched healthy controls. Genotypes of rs7903146 (C/T) SNP in the TCF7L2 gene were evaluated by real-time polymerase chain reaction via TaqMan allelic discrimination. Logistic regression was used to detect the most independent factor for development of diabetes and diabetic microvascular complications. Variant homozygous TT and heterozygous CT genotypes were significantly increased in diabetic without complications and diabetic with complications groups than controls (p = 0.003, 0.001) respectively. The T allele was more represented in both patient groups than controls with no significant difference between patient groups. TT genotype as well as T allele was significantly associated with increased T2DM risk.

Conclusion

The T allele of rs7903146 polymorphism of TCF7L2 confers susceptibility to development of T2DM. However, no significant association was found for diabetic complications.

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Data availability

The datasets generated analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

2 h pp:

2 Hours Postprandial

ACR:

Microalbumin/Creatinine Ratio

DN:

Diabetic Nephropathy

DR:

Diabetic Retinopathy

GLP-1:

Glucagon-Like Peptide 1

HbA1c:

Glycated Hemoglobin

HDL-C:

High-Density Lipoprotein

LDL-C:

Low-Density Lipoprotein

T2DM:

Type 2 Diabetes Mellitus

TCF7L2:

Transcription Factor 7-Like 2

TG:

Triglycerides

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Funding

No funding was received for conducting this study.

Author information

Authors and Affiliations

  1. Clinical Pathology Department, Faculty of Medicine, Menoufia University, Shibīn al-Kawm, Egypt

    Noran Talaat Aboelkhair & Rawhia Hassan El-Edel

  2. Internal Medicine Department, Faculty of Medicine, Menoufia University, Shibīn al-Kawm, Egypt

    Heba Elsayed Kasem

  3. Menoufia Co-Regional Lab, Menoufia Ministry of Health, Shibīn al-Kawm, Egypt

    Amera Anwar Abdelmoaty

Authors
  1. Noran Talaat Aboelkhair
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  2. Heba Elsayed Kasem
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  3. Amera Anwar Abdelmoaty
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  4. Rawhia Hassan El-Edel
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Contributions

Conceptualization: NTA, RHE-E. Methodology: NTA, AAAE-M. Formal analysis and investigation: AAAE-M, HEK. Writing-original draft preparation: AAAE-M. Writing—review and editing: NTA. Funding acquisition: No fund. Supervision: RHE-E. All authors have read and approved the manuscript.

Corresponding author

Correspondence to Noran Talaat Aboelkhair.

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Conflict of interest

The authors have no conflicts of interest to declare that are relevant to the content of this article.

Consent to participate

Consent to participate was obtained from all individuals included in the study.

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Consent to publish relevant data was taken from all participants.

Ethical approval

This research was approved by the Research Ethics Committee at Menoufia Faculty of Medicine according to 1964 Helsinki declaration and informed consent was taken from every participant in the study. IRB approval number: 19919CPATH31.

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Aboelkhair, N.T., Kasem, H.E., Abdelmoaty, A.A. et al. TCF7L2 gene polymorphism as a risk for type 2 diabetes mellitus and diabetic microvascular complications. Mol Biol Rep 48, 5283–5290 (2021). https://doi.org/10.1007/s11033-021-06537-0

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