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Involvement of Rac1 in macrophage activation by brain-derived neurotrophic factor

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Abstract

Brain-derived neurotrophic factor (BDNF) enhances periodontal tissue regeneration. Tissue regeneration is characterized by inflammation, which directs the quality of tissue repair. This study aimed to investigate the effect of BDNF on the phagocytic activity of RAW264.7 cells. In addition, we studied the effect of BDNF on guanosine triphosphatase (GTP)-RAS-related C3 botulinus toxin substrate (Rac)1 and phospho-Rac1 levels in RAW264.7 cells. Rac1 inhibitor inhibited BDNF-induced phagocytosis of latex-beads. In addition, BDNF enhanced Porphyromonas gingivalis (Pg) phagocytosis by RAW264.7 cells as well as latex-beads. We demonstrated for the first time that BDNF enhances phagocytic activity of RAW264.7 cells through Rac1 activation. The present study proposes that BDNF may reduce inflammatory stimuli during BDNF-induced periodontal tissue regeneration through enhanced phagocytic activity of macrophages.

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Acknowledgements

This work was supported by JSPS KAKENHI Grants JP25463218 [Grant-in-Aid for scientific Reserch (C)]. We thank the Analysis Center of Life Science of Hiroshima University for the use of facilities.

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Authors and Affiliations

  1. Department of Periodontal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, 734-8553, Japan

    Shinya Sasaki, Kazuhisa Ouhara, Mikihito Kajiya, Shinji Matsuda, Shoko Kono, Hidemi Kurihara & Noriyoshi Mizuno

  2. Department of Biological Endodontics, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, 734-8553, Japan

    Katsuhiro Takeda, Satomi Shirawachi & Hideki Shiba

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  1. Shinya Sasaki
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  2. Katsuhiro Takeda
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  3. Kazuhisa Ouhara
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  4. Satomi Shirawachi
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All authors equally contributed and gave their final approval to the submitted manuscript.

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Correspondence to Katsuhiro Takeda.

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Sasaki, S., Takeda, K., Ouhara, K. et al. Involvement of Rac1 in macrophage activation by brain-derived neurotrophic factor. Mol Biol Rep 48, 5249–5257 (2021). https://doi.org/10.1007/s11033-021-06531-6

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  • DOI: https://doi.org/10.1007/s11033-021-06531-6

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