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Increased insulin and GLUT2 gene expression and elevated glucokinase activity in β-like cells of islets of langerhans differentiated from human haematopoietic stem cells on treatment with Costus igneus leaf extract


In the quest to understand lost β-cells regeneration in the diabetic condition, we have demonstrated successful differentiation of human haematopoietic stem cells (HSCs) to functional β-like cells. Costus igneus (Ci) leaf extract is known to exhibit anti-diabetic properties by lowering the blood glucose level as demonstrated in mice models. To establish the anti-diabetic properties of Ci leaf extract on human subjects, we studied the effect of Ci on these differentiated β-like cells. Ci leaf extract showed its anti-diabetic property through elevated glucokinase activity which catalyzes the rate-limiting step of glucose catabolism in β-like cells and acts as a sensor for insulin production while decreasing the glucose-6-phosphatase activity. Upon increasing the concentrations of Ci leaf extract (25, 65, 105, 145, 185 µg/ml) and glucose concentrations (5.5, 11.1, and 25 mM) Ci leaf extract treated β-like cells showed enhanced glucokinase and decreased glucose-6-phosphatase activities and an exponential rise in gene expressions of INS and GLUT2 was observed. The present study shows enhanced INS and GLUT2 gene expression and elevated glucokinase activity in β-like cells differentiated from HSCs upon treatment with Ci leaf extract explain the anti-diabetic property of Ci leaf extract. This extract can be effectively used in the management of diabetes.

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We acknowledge Sri Venkateswara Institute of Medical Sciences for providing facilities to carry out the work.

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Authors and Affiliations



Work design editing of manuscript: PVGKS. Treating of Costus igneus to in vitro differentiated β-like cells, Writing of Manuscript: SK. Characterization of in vitro differentiated β-like cells: SMM. Characterization of Human HSCs: SEL, SK.

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Correspondence to Venkata Gurunadha Krishna Sarma Potukuchi.

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The Institutional Ethics committee approved the research protocol (IEC No: 31/06/2006 and 419/27–01-2015).

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Blood donor has given written informed consent for his participation in the study.

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Kattaru, S., Manne Mudhu, S., Echambadi Loganathan, S. et al. Increased insulin and GLUT2 gene expression and elevated glucokinase activity in β-like cells of islets of langerhans differentiated from human haematopoietic stem cells on treatment with Costus igneus leaf extract. Mol Biol Rep 48, 4477–4485 (2021).

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  • In vitro differentiated β-like cells
  • Human Haematopoietic stem cells
  • Costus igneus
  • Anti-diabetic property