Abstract
Diabetes mellitus (DM) is a metabolic disease characterized by hyperglycemia and abnormal insulin secretion. MicroRNAs are small, non-coding RNAs that are able to affect cell biological functions and act as biomarkers for some diseases such as DM. In current study, we measured serum miR-33 in three groups (n = 15) as follows; non-diabetic control, pre-diabetic, and DM patients. Real-time PCR method was used to quantify miR-33 expression. miR-33 expression was significantly increased in pre-diabetic subjects compared to other two groups (p < 0.001). FBS (p < 0.001), insulin (p < 0.001), HOMA-IR (p < 0.001), and TG (p = 0.026) were higher in diabetic subjects than the other two groups. In people that had high physical activity, the number of diabetic subjects were zero and most of them were in pre-diabetic group (p = 0.019). Serum miR-33 level significantly and positively correlated with pre-diabetic state (B = 2.67, p = 0.000), Sex (B = 1.03, p = 0.025), and FBS (B = 0.04, p = 0.036) and also miR-33 was significantly and negatively correlated with HOMA-IR (B = − 1.58, p = 0.04). These findings support the possible role of miR-33 to monitor pre-diabetes onset and progression. It needs to be evaluated in future studies with high number of participants to clarify its mechanism and diagnostic viability.
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The data that support the findings of this study are available from the corresponding author, [YMA], upon reasonable request.
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This research was financially supported by Kerman University of Medical Sciences Research Council and Physiology Research Center.
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This research was financially supported by Kerman University of Medical Sciences Research Council.
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All authors contributed to the study conception and design, data collection and analysis. The authors declare that all data were generated in-house and that no paper mill was used. Experimental procedures were performed by BS, HF, SA and YM-A. The first draft of the manuscript was written by BS and ME and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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This study was approved by the Ethics Committee of Kerman University of Medical Sciences (The Ethic approval Cod is IR.KMU.REC.1396.2537) and all procedures of the current study was in accordance with standards set by the Declaration of Helsinki.
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Shahouzehi, B., Eghbalian, M., Fallah, H. et al. Serum microRNA-33 levels in pre-diabetic and diabetic patients. Mol Biol Rep 48, 4121–4128 (2021). https://doi.org/10.1007/s11033-021-06425-7
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DOI: https://doi.org/10.1007/s11033-021-06425-7