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Transferability and characterization of microsatellite markers from Byrsonima cydoniifolia A. Juss. (MALPIGHIACEAE) in seven related taxa from Cerrado biome reveal genetic relationships

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Abstract

Byrsonima Rich. is one of the largest genera of the Malpighiaceae family with 97 species occurrence in Brazil and multiple potentialities, including pharmaceutical and food industries. In this study, 17 microsatellite markers characterized in Byrsonima cydoniifolia were tested for seven related taxa, all species are native to Brazil and four are endemic. Genomic DNA was extracted from leaves tissues and 17 microsatellite markers were used to cross-amplification of microsatellite regions. Polymorphism and genetic diversity were evaluated for B. intermedia, B. verbascifolia, B. laxiflora, B. subterranea, B. umbellata, B. linearifolia. from 16 individuals and for B. viminifolia from 14 individuals. Transferred microsatellite markers panels ranged from 11 (64.8%) in B. viminifolia to 6 (35.2%) in B. umbellata. The total number of alleles per locus ranged from 5 (B. linearifolia) to 8 (B. subterranea) alleles. B. umbellata showed lower values of observed and expected heterozygosity (HO = 0.312; HE = 0.436) and B. subterranea presented the highest values (HO = 0.687; HE = 0.778). A greater number of microsatellite markers should be developed for B. umbellata. The microsatellite marker panels transferred to the species B. intermedia, B. verbascifolia, B. laxiflora, B. subterranea, B. viminifolia and B. linearifolia are very informative, with a high combined probability of exclusion of paternity (Q ≥ 0.976) and the low combined probability of identity (I ≤ 9.91 × 10–6), potentially suitable for future genetic-population studies, supporting strategies for maintaining the genetic diversity and for exploration of Byrsonima species as genetic resources.

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Acknowledgements

This work was supported by a PRONEX project from: PRONEX—FAPEG/CNPq (CP 07/2012). We would like to thank the reviewers for all the suggestions and the consequent improvement of our manuscript. The Coordination for the Improvement of Higher Education Personnel (CAPES) for the doctoral scholarship and the National Council for Scientific. M.P.C.T. have been continuously supported by productivity fellowships from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Our current research in Genetics and Genomics is developed in the context of National Institutes for Science and Technology (INCT) in Ecology, Evolution and Biodiversity Conservation, supported by MCTIC/CNPq (Proc. 465610/2014-5) and Fundação de Amparo à Pesquisa do Estado de Goiás (FAPEG), which we gratefully acknowledge.

Funding

Work by V. Bernardes was supported by a fellowship from CAPES, and work by M. P. C. Telles, was also supported by productivity grants from CNPq. This study was partially supported by a Programa de Apoio a Núcleos de Excelência (PRONEX) grant from CNPq/FAPEG (AUX PESQ CH 007/2009) and by grants to the research network GENPAC (Geographical Genetics and Regional Planning for natural resources in Brazilian Cerrado) from CNPq/FAPEG (Project No. 201110267000125 and 563839/ 2010-4–563973/2010-2). Our current research in Genetics and Genomics is developed in the context of National Institutes for Science and Technology (INCT) in Ecology, Evolution and Biodiversity Conservation, supported by MCTIC/CNpq (Proc. 465610/2014-5) and FAPEG.

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Authors and Affiliations

  1. Laboratory of Genetics and Biodiversity, Institute of Biological Sciences, Universidade Federal de Goiás, Goiânia, Goiás, 74690-900, Brazil

    Vanessa Bernardes, Tamara N. Souza & Mariana P. C. Telles

  2. Institute of Exact and Earth Sciences, Universidade Federal de Mato Grosso, Barra do Garças, Mato Grosso, 78600-000, Brazil

    Devanir M. Murakami

  3. Institute of Biological Science and Health, Universidade Federal de Mato Grosso, Barra do Garças, Mato Grosso, 78600-000, Brazil

    Nair Bizão

  4. Plant Morphology and Taxonomy Laboratory, Department of Botany, Institute of Biological Sciences, Universidade Federal de Goiás, Goiânia, Goiás, 74690-900, Brazil

    Marcos J. da Silva

  5. College of Agriculture and Biological Sciences, Pontifícia Universidade Católica de Goiás, Goiânia, Goiás, 74605-010, Brazil

    Mariana P. C. Telles

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  1. Vanessa Bernardes
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Contributions

DMM, NB, MJS; Marcos José provided the plant material. MPCT, DMM and NB conceived the idea and provide necessary resources. VB and TNS designed the experiments, mined the data and perform the experiments. VB and MPCT wrote the manuscript. All authors have read and approved the final manuscript.

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Correspondence to Vanessa Bernardes.

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Bernardes, V., Murakami, D.M., Bizão, N. et al. Transferability and characterization of microsatellite markers from Byrsonima cydoniifolia A. Juss. (MALPIGHIACEAE) in seven related taxa from Cerrado biome reveal genetic relationships. Mol Biol Rep 48, 4039–4046 (2021). https://doi.org/10.1007/s11033-021-06411-z

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