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Effect of methylenetetrahydrofolate reductase gene polymorphisms and oxidative stress in silent brain infarction

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Abstract

Ischemic infarctions occur under the influence of genetic and environmental factors. In our study, the role of ischemia-modified albumin and thiol balance, which are new markers in determining oxidative damage together with MTHFR gene polymorphisms and homocysteine levels, in the development of SBI was investigated. White matter lesions in the magnetic resonance imaging (MRI) results of the patients were evaluated according to the Fazekas scale and divided into groups (Grade 0, 1, 2, and 3). Homocysteine, folate, B12, IMA, total thiol, and native thiol were measured by biochemical methods. The polymorphisms in MTHFR genes were investigated by the RT-PCR method. According to our results, a significant difference was found between the groups in age, homocysteine, folate, IMA, total thiol, and native thiol parameters (p < 0.05). When we compared the groups in terms of genotypes of the C677T gene, we found a significant difference in TT genotype between grades 0/3 and 1/3 (p < 0.05). We determined that homocysteine and IMA levels increased and folate levels decreased in CC/TT and CT/TT genotypes in the C677T gene (p < 0.05). Considering our results, the observation of homocysteine and IMA changes at the genotype level of the MTHFR C677T gene and between the groups, and the deterioration of thiol balance between the groups suggested that these markers can be used in the diagnosis of silent brain infarction.

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Authors and Affiliations

  1. Department of Medical Biology, University of Süleyman Demirel, Isparta, Turkey

    Pınar Aslan Koşar

  2. Department of Medical Genetics, University of Süleyman Demirel, Isparta, 32300, Turkey

    Muhammet Yusuf Tepebaşı

  3. Department of Neurology, University of Süleyman Demirel, Isparta, Turkey

    Nihat Şengeze & Süleyman Kutluhan

  4. Department of Medical Biochemistry, University of Süleyman Demirel, Isparta, Turkey

    İlter İlhan & Halil İbrahim Büyükbayram

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  1. Pınar Aslan Koşar
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  4. İlter İlhan
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All of the authors contributed to the design of the study, collection of samples, and analysis and interpretation of data.

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Correspondence to Muhammet Yusuf Tepebaşı.

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Dr. Pınar ASLAN KOŞAR and the co-authors have no conflicts of interest to declare in association with this study.

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This study was performed in accordance with Declaration of Helsinki ethics guidelines, and informed consent was obtained from each of its participants. Protocol and informed consent forms was approved by Isparta Süleyman Demirel University Medical Faculty Ethics Committee (Dated:27 February 2020, No: 42423).

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Aslan Koşar, P., Tepebaşı, M.Y., Şengeze, N. et al. Effect of methylenetetrahydrofolate reductase gene polymorphisms and oxidative stress in silent brain infarction. Mol Biol Rep 48, 3955–3962 (2021). https://doi.org/10.1007/s11033-021-06395-w

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