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Comparative analysis of the immunomodulatory potential of caprine fetal adnexa derived mesenchymal stem cells

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Abstract

The caprine mesenchymal stem cells (MSCs) derived from fetal adnexa are highly proliferative. These cells possess tri-lineage differentiation potential and express MSC surface antigens and pluripotency markers with a wound-healing potential. This present study was conducted to compare the immunomodulatory potential of caprine MSCs derived from the fetal adnexa. Mid-gestation caprine uteri (2–3 months) were collected from the abattoir to isolate MSCs from amniotic fluid (cAF), amniotic sac (cAS), Wharton’s jelly (cWJ) and cord blood (cCB), which were expanded and characterized at the 3rd passage. These MSCs were then stimulated with inflammatory cytokines (IFN-γ and TNF-α) to assess the percentage of inhibition produced on peripheral blood mononuclear cells (PBMCs) proliferation. The percentage of inhibition on activated PBMCs proliferation produced by cWJ MSCs and cAS MSCs was significantly higher than cCB and cAF MSCs. The relative mRNA expression profile and immunofluorescent localization of different immunomodulatory cytokines and growth factors were conducted upon stimulation. The mRNA expression profile of a set of different cytokines and growth factors in each caprine fetal adnexa MSCs were modulated. Indoleamine 2, 3 dioxygenase appeared to be the major immunomodulator in cWJ, cAF, and cCB MSCs whereas inducible nitric oxide synthase in cAS MSCs. This study suggests that caprine MSCs derived from fetal adnexa display variable immunomodulatory potential, which appears to be modulated by different molecules among sources.

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Acknowledgement

The authors are thankful to the Director, ICAR-Indian Veterinary Research Institute, Izatnagar, for providing necessary facilities for conducting this work.

Funding

This study was supported by ICAR-IVRI through the Flagship Project entitled ‘Stem cells: its biology and therapeutic application in livestock and pets’. The funders had no role in study design.

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Authors and Affiliations

  1. Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, U.P, India

    Anjali Somal, Irfan A. Bhat, Sriti Pandey, Mohd Matin Ansari, Bibhudatta S. K. Panda, Mukesh Kumar Bharti, Vikash Chandra & G. Taru Sharma

  2. Division of Veterinary Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, U.P, India

    G. Saikumar

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  1. Anjali Somal
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  2. Irfan A. Bhat
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  4. Mohd Matin Ansari
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  5. B. Indu
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  6. Bibhudatta S. K. Panda
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Contributions

Conceived and designed the experiments: G Taru Sharma and Vikash Chandra. Performed the experiments: Anjali Somal, Irfan A. bhat, Mukesh Kumar Bharti, Bibhudatta S. K. Panda. Analyzed the data: Anjali Somal, Sriti Pandey and Irfan A. bhat. Mohd Matin Ansari Contributed reagents/materials/analysis tools: G Taru Sharma, Vikash Chandra and G Saikumar. Wrote the paper: Anjali Somal, Mukesh Kumar Bharti, Sriti Pandey, B. Indu, Irfan A. bhat, G Taru Sharma.

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Correspondence to G. Taru Sharma.

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Somal, A., Bhat, I.A., Pandey, S. et al. Comparative analysis of the immunomodulatory potential of caprine fetal adnexa derived mesenchymal stem cells. Mol Biol Rep 48, 3913–3923 (2021). https://doi.org/10.1007/s11033-021-06383-0

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