Abstract
Gene expression can be modulated by epigenetic modifications, which may lead to a rapid adaptation to environmental stress. After stress cessation, changes in gene expression could be reversed, which would allow organisms to maintain their phenotype under transient environments, but this mechanism is poorly understood. Social stress downregulates a gene directly involved in pheomelanin synthesis (Slc7a11) by changing DNA m5C levels, avoiding cellular damage caused by stress. We thus investigated if Slc7a11 expression is reversed in melanocytes of growing flank feathers to avoid changes in the pigmentation phenotype. We measured the expression level of Slc7a11 at three time points: before stress exposure, immediately after stress exposure and six weeks after stress cessation in 37 male zebra finches (Taeniopygia guttata). No differences in Slc7a11 expression were detected between birds exposed to stress and controls six weeks after stress elimination, indicating that stress removal led to a cessation of Slc7a11 downregulation. Reversibility in Slc7a11 expression, probably mediated by reversible changes in DNA methylation, may thus avoid altering the pigmentation phenotype during transient stressful conditions. This is one of the few studies in vertebrates supporting the idea that reversible gene expression responses allow organisms adapting to changing environmental conditions.
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Acknowledgements
We thank Sara Borrego for help with laboratory work. This work was supported by Spain’s Ministry of Science, Innovation and Universities: Project CGL2015‐67796‐P, Ramón y Cajal Fellowship RYC‐2012‐10237 to I.G., and Grant BES‐2016‐077112 to S.R‐M.
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SRM and IG conceived the study and wrote the manuscript. SRM conducted the experiment and performed laboratory and statistical analysis.
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This study was approved by the Bioethics Subcommittee of Consejo Superior de Investigaciones Cientificas (CSIC; Ref. 651/2018) and local authorities (Consejería de Agricultura, Pesca y Medio Ambiente, Junta de Andalucía; Ref. 23/02/2018/016).
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Rodríguez-Martínez, S., Galván, I. Slc7a11 downregulation is rapidly reversed after cessation of competitive social stress in zebra finches. Mol Biol Rep 48, 3007–3010 (2021). https://doi.org/10.1007/s11033-021-06256-6
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DOI: https://doi.org/10.1007/s11033-021-06256-6