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Assessment of IL-10, IL-1ß and TNF-α gene polymorphisms in patients with peri-implantitis and healthy controls

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Abstract

Peri-implantitis (PI) is a multifactorial condition caused by the interactions of pathogens and the host immune response. Previous studies have demonstrated a relationship between PI and specific gene polymorphisms, particularly cytokine genes involved in the pathogenesis of PI. This study aimed to evaluate the frequency of single nucleotide polymorphisms (SNPs) of interleukin-10 (IL-10), interleukin 1ß (IL-1ß), and tumor necrosis factor-α (TNF-α) genes in PI patients and healthy controls. A total of 50 patients with PI and 89 periodontally healthy controls were recruited for this study. Venous blood samples (5 cc) were collected, and DNA was extracted. After DNA purification, the relevant gene segments were amplified by polymerase chain reaction (PCR). Restriction fragment length polymorphism (RFLP) and electrophoresis were performed to assess the polymorphisms of the related genes. The analysis revealed that allele and genotype frequencies of IL-10 ─ 819 C/T, IL-10 ─ 592 C/A, and IL-1ß + 3954 C/T significantly differed between PI patients and healthy controls. The analysis revealed no significant association between TNF-α ─ 857 G/A and TNF-α ─ 308 G/A polymorphisms and PI. Our results indicated that specific gene polymorphisms of IL-10 ─ 819 C/T, IL-10 ─ 592 C/A, and IL-1ß + 3954 C/T may play a role in the pathogenesis of PI, and increase its risk of occurrence.

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Funding

This work was supported by a grant from Shahid Beheshti University of Medical Science Grant No. 0000.

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Authors and Affiliations

  1. Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Leila Saremi, Marziyeh Shafizadeh & Mahdi Kadkhodazadeh

  2. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Leila Saremi

  3. School of Medicine, Aja University of Medical Sciences, Tehran, Iran

    Emran Esmaeilzadeh

  4. Dental Sciences and Research Center, Faculty of Dentistry, Guilan University of Medical Sciences, Rasht, Iran

    Mohammad Ebrahim Ghaffari

  5. Periodontics Department, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Mohammad hosein Mahdavi & Reza Amid

Authors
  1. Leila Saremi
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  2. Marziyeh Shafizadeh
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  3. Emran Esmaeilzadeh
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  4. Mohammad Ebrahim Ghaffari
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  5. Mohammad hosein Mahdavi
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  7. Mahdi Kadkhodazadeh
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All the authors have substantial contributions to the conception or design of the work, revising and drafting the work.

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Correspondence to Mahdi Kadkhodazadeh.

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This work was approved by ethical committee of Shahid Beheshti University of Medical Science.

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Saremi, L., Shafizadeh, M., Esmaeilzadeh, E. et al. Assessment of IL-10, IL-1ß and TNF-α gene polymorphisms in patients with peri-implantitis and healthy controls. Mol Biol Rep 48, 2285–2290 (2021). https://doi.org/10.1007/s11033-021-06253-9

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  • DOI: https://doi.org/10.1007/s11033-021-06253-9

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