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Impact of heat stress responsive factors on growth and physiology of cotton (Gossypium hirsutum L.)

Molecular Biology Reports volume 48, pages 1069–1079 (2021)Cite this article

Abstract

Pakistan ranked highest with reference to average temperatures in cotton growing areas of the world. The heat waves are becoming more intense and unpredictable due to climate change. Identification of heat tolerant genotypes requires comprehensive screening using molecular, physiological and morphological analysis. Heat shock proteins play an important role in tolerance against heat stress. In the current study, eight heat stress responsive factors, proteins and genes (HSFA2, GHSP26, GHPP2A, HSP101, HSC70-1, HSP3, APX1 and ANNAT8) were evaluated morphologically and physiologically for their role in heat stress tolerance. For this purpose, cotton crop was grown at two temperature conditions i.e. normal weather and heat stress at 45 °C. For molecular analysis, genotypes were screened for the presence or absence of heat shock protein genes. Physiological analysis of genotypes was conducted to assess net photosynthesis, stomatal conductance, transpiration rate, leaf-air temperature and cell membrane stability under control as well as high temperature. The traits photosynthesis, cell membrane stability, leaf-air temperature and number of heat stress responsive factors in each genotypes showed a strong correlation with boll retention percentage under heat stress. The genotypes with maximum heat shock protein genes such as Cyto-177, MNH-886, VH-305 and Cyto-515 showed increased photosynthesis, stomatal conductance, negative leaf-air temperature and high boll retention percentage under heat stress condition. These varieties may be used as heat tolerant breeding material.

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Acknowledgements

The study was part of the NRPU research project of HEC entitled “Marker assisted gene pyramiding for heat tolerance in cotton” Project ID: 7965. We are thankful to HEC for providing funds for this research work.

Funding

Source: Higher Education Commission Islamabad, Pakistan.

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Affiliations

  1. Department of Plant Breeding and Genetics, Bahauddin Zakaryia University, Multan, Pakistan

    Muhammad Asif Saleem, Waqas Malik, Abdul Qayyum, Muhammad Qadir Ahmad, Hammad Afzal & Muhammad Farjad Ateeq

  2. College of Agriculture, Bahauddin Zakariya University, Bahadur Sub-campus, Layyah, Pakistan

    Sami Ul-Allah

  3. State Key Lab. of Crop Genetics & Germplasm, Nanjing Agriculture University, Nanjing, China

    Muhammad Waqas Amjid

  4. Cotton Research Institute, Multan, Pakistan

    Zia Ullah Zia

Authors
  1. Muhammad Asif Saleem
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  2. Waqas Malik
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  3. Abdul Qayyum
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  4. Sami Ul-Allah
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  5. Muhammad Qadir Ahmad
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  6. Hammad Afzal
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  7. Muhammad Waqas Amjid
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  8. Muhammad Farjad Ateeq
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  9. Zia Ullah Zia
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Contributions

MAS (PI), WM (Co-PI), AQ (Supervised Field work), SUA (data analysis), MQA (Supervised lab work), HA (Field work), MWA (manuscript preparation), MFA (Lab work) and ZUZ (Checked purity of research material in the field).

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Correspondence to Muhammad Asif Saleem.

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Saleem, M.A., Malik, W., Qayyum, A. et al. Impact of heat stress responsive factors on growth and physiology of cotton (Gossypium hirsutum L.). Mol Biol Rep 48, 1069–1079 (2021). https://doi.org/10.1007/s11033-021-06217-z

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  • DOI: https://doi.org/10.1007/s11033-021-06217-z

Keywords

  • Cotton
  • Climate change
  • Heat shock proteins
  • Photosynthesis
  • Transpiration