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CREB-binding protein (CREBBP) and preeclampsia: a new promising target gene

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Abstract

Preeclampsia (PE) is a major complication of pregnancy and remains a leading cause of neonatal and maternal mortality worldwide. Several studies have revealed that the incidence of preeclampsia is high in mothers who carried a fetus with Rubinstein-Taybi Syndrome due to the mutation in CREBBP. We aimed to compare the expression level of the CERBBP gene between preeclamptic and healthy placenta in our study. The expression level of CREBBP gene was evaluated in a total of one hundred placental biopsies from PE patients and healthy pregnant women after delivery using quantitative real-time polymerase chain reaction (qRT-PCR). Moreover, the differential expression of CREBBP was assessed between the maternal and fetal sides of the placenta. Expression of the CREBBP gene was higher in preeclampsia patients compared with the controls (Fold change = 2.158; P = 0.018). Moreover, the gene expression was slightly higher in the fetal side of the placenta, although it was not significantly different (Fold change = 1.713, P = 0.254). Our findings show a role for CREBBP in the pathogenesis of PE. Due to the important role of CREBBP in angiogenesis and hypoxia, the gene may serve as a promising target in future studies.

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Acknowledgements

The authors gratefully acknowledge all participants for their collaboration in this research. This study was financially supported by the “Research Department of the School of Medicine Shahid Beheshti University of Medical Sciences” (Pajoohan code: 22424).

Funding

This study was funded by the “Research Department of the School of Medicine, Shahid Beheshti University of Medical Sciences” (Grant Number: 22424).

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Author notes

  1. Hossein Sadeghi, Sahra Esmkhani, Reihaneh Pirjani, and Mona Amin-Beidokhti equally contributed to this study.

Authors and Affiliations

  1. Genomic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Hossein Sadeghi & Reza Mirfakhraie

  2. Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Koodakyar St., Velenjak Ave, Chamran Highway, Tehran, Iran

    Sahra Esmkhani, Mona Amin-Beidokhti, Ghasem Azizi Tabesh, Mohammad Reza Ghasemi & Reza Mirfakhraie

  3. Department of Cancer Genetics, Oncology Institute, Istanbul University, Istanbul, Turkey

    Sahra Esmkhani

  4. Obstetrics and Gynecology Department, Arash Women Hospital, Tehran University of Medical Sciences, Tehran, Iran

    Reihaneh Pirjani

  5. Department of Biochemistry and Genetics, School of Medicine, Arak University of Medical Sciences, Arak, Iran

    Milad Gholami

  6. Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Latif Gachkar

Authors
  1. Hossein Sadeghi
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  2. Sahra Esmkhani
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Contributions

Authors participated in conception and design (HS, RP, LG, RM), clinical data collection and statistical analysis (RP, SE, MRG, GAT), performing molecular experiments (SE, GAT and MAB), and drafting the article or revising critically for important intellectual content (HS, MG and RM).

Corresponding author

Correspondence to Reza Mirfakhraie.

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The authors declare that they have no conflict of interest.

Ethical approval

This study was in accordance with the ethical standards of the ethics committee of Shahid Beheshti University of Medical Sciences and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Ethical approval was obtained from Shahid Beheshti University of Medical Sciences ethics committee (Code No: IR.SBMU.RETECH.REC.1399.99).

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All subjects signed the informed consent form prior to the study.

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Sadeghi, H., Esmkhani, S., Pirjani, R. et al. CREB-binding protein (CREBBP) and preeclampsia: a new promising target gene. Mol Biol Rep 48, 2117–2122 (2021). https://doi.org/10.1007/s11033-021-06215-1

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  • DOI: https://doi.org/10.1007/s11033-021-06215-1

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