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Knock-down of glucose transporter and sucrose non-fermenting gene in the hemibiotrophic fungus Colletotrichum falcatum causing sugarcane red rot

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Abstract

Red rot caused by Colletotrichum falcatum, is one of the economically important disease of sugarcane and breeding for resistant varieties is considered to be the major solution to manage the disease. However, breakdown of red rot resistance become usual phenomenon due to development of newer races by culture adaptation on newly released varieties. Hence it is needed to characterize the genes responsible for pathogen virulence in order to take care of host resistance or to manage the disease by other methods. The transcript studies gave foundation to characterize the huge number of pathogenicity determinants and their role in pathogenesis. Here we studied role of two important genes viz., Glucose Transporter (GT) and Sucrose Non-Fermenting1 (SNF1) during pathogenesis of C. falcatum, which said to be involved in carbon source metabolism. Sugar metabolism has a vital role in disease progression of C. falcatum by regulating their cell growth, metabolism and development of the pathogen during various stages of infection. The present study was aimed to find out the role of GT and SNF1 genes in response to pathogenicity by RNA silencing (RNAi) approach. Knock-down of the target pathogenicity gene homologs in standard C. falcatum isolate Cf671 was carried out by amplifying sense and antisense fragments of targets individually using pSilent-1 vector. The expression cassette was cloned into the binary vector pCAMBIA1300 followed by fungal transformation through Agarobacterium mediated transformation. Resulted mutants of both the genes showed less virulence compared to wild type isolate. Simultaneously, both the mutants did not produce spores. Moreover, the molecular confirmation of the mutants displayed the expression of hygromycin gene with reduced expression of the target gene during host-pathogen interaction. Knockdown of the pathogenicity related genes (GT and SNF1) by RNAi approach corroborate the possible role of the genes in causing the disease.

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References

  1. Ali SS, Nugent B, Mullins E, Doohan FM (2013) Insights from the fungus Fusarium oxysporium point to high affinity glucose transporters as targets for enhancing ethanol production from lignocelluloses. PLoS One 8:e54701

    Article  CAS  Google Scholar 

  2. Carlson M, Osmond BC, Botstein D (1981) Mutants of yeast defective in sucrose utilization. Genet 98:25–40

    Article  CAS  Google Scholar 

  3. Dickman MB, Yarden O (1999) Serine/threonine protein kinases and phosphatases in filamentous fungi. Fungal Genet Biol 26:99–117

    Article  CAS  Google Scholar 

  4. Gancedo JM (1998) Yeast carbon catabolite repression. Microbiol Mol Biol R 62(2):334–361

    Article  CAS  Google Scholar 

  5. Goodwin PH, Chen GY (2002) High expression of a sucrose non-fermenting (SNF1)-related protein kinase from Colletotrichum gloeosporoides f. sp. malvae is associated with penetration of Malva pusilla1. FEMS Microbiol Lett 215:169–174

    Article  CAS  Google Scholar 

  6. Goodwin PH, Li J, Jin S (2000) Evidence for sulfate derepression of an arylsulfatase gene of Colletotrichum gloeosporoides f.sp. malvae during infection of round-leaved mallow, Malva pusilla. Physiol Mol Plant Pathol 57:169–176

    Article  CAS  Google Scholar 

  7. Hardie DG, Carling D, Carlson M (1998) The AMP-activated/ANF1 kinase subfamily: metabolic sensors of the eukaryotic cell? Annu Rev Biochem 67:821–855

    Article  CAS  Google Scholar 

  8. He PH, Wang XX, Chu XL et al (2015) RNA sequencing analysis identifies the metabolic and developmental genes regulated by BbSNF1 during conidiation of the entomopathogenic fungus Beauveria bassiana. Curr Genet 61:143–152

    Article  Google Scholar 

  9. Hunter T, Plowman GD (1997) The protein kinases of budding yeast: six score and more. Trends Biochem Sci 22:18–22

    Article  CAS  Google Scholar 

  10. Islam KT, Bond JP, Fakhoury AM (2017) FvSNF1, the sucrose non-fermenting protein kinase gene of Fusarium virguliforme, is required for cell-wall-degrading enzymes expression and sudden death syndrome development in soybean. Curr Genet 63:723–738

    Article  CAS  Google Scholar 

  11. Karunakaran M, Nair V, Rho HS, Choi J, Kim S, Lee YH (2008) Agrobacterium tumefaciens-mediated transformation in Colletotrichum falcatum and C. acutatum. J Microbiol Biotechnol 18(2):234–241

    Google Scholar 

  12. Kim JH, Polish J, Johnston M (2003) Specificity and regulation of DNA binding by the yeast glucose transporter gene repressor Rgt1. Mol Cell Biol 23:5208–5216

    Article  CAS  Google Scholar 

  13. Krijger JJ, Horbach R, Behr M, Schweizer P, Deising HB, Wirsel SG (2008) The yeast signal sequence trap identifies secreted proteins of the hemibiotrophic corn pathogen Colletotrichum graminicola. Mol Plant-Microbe Interact 21:1325–1336

    Article  CAS  Google Scholar 

  14. Lee SH, Lee J, Lee S, Park EH, Kim KW (2009) GzSNF1 is required for normal sexual and asexual development in the ascomycete Gibberellazeae. Eukaryot Cell 8:116–127

    Article  CAS  Google Scholar 

  15. Lingner U, Münch S, Deising HB, Sauer N (2011) Hexose transporters of a hemibiotrophic plant pathogen: functional variations and regulatory differences at different stages of infection. J Biol Chem 286(23):20913–20922

    Article  CAS  Google Scholar 

  16. Mendgen K, Hahn M (2002) Plant infection and the establishment of fungal biotrophy. Trends Plant Sci 7:352–356

    Article  CAS  Google Scholar 

  17. Ming Y, Wei Q, Jin K, Xia Y (2014) MaSnf1, a sucrose non-fermenting protein kinase gene, is involved in carbon source utilization, stress tolerance, and virulence in Metarhizium acridum. Appl Genet Mol Biotechnol 98:10153–10164

    CAS  Google Scholar 

  18. Munch S, Lingner U, Floss D, Ludwig N, Sauer N, Deising HB (2008) The hemibiotrophic lifestyle of Colletotrichum species. J Plant Physiol 165:41–51

    Article  Google Scholar 

  19. Ospina-Giraldo MD, Mullins E, Kang S (2003) Loss of function of the Fusarium oxysporum SNF1 gene reduces virulence on cabbage and Arabidopsis. Curr Genet 44:49–57

    Article  CAS  Google Scholar 

  20. Ozcan S, Johnston M (1999) Function and regulation of yeast hexose transporters. Microbiol Mol Biol R 63:554–569

    Article  CAS  Google Scholar 

  21. Pereira MF, Santos CM, Araujo EF, Queiroz MV, Bazzolli DM (2013) Beginning to understand the role of sugar carriers in Colletotrichum lindemuthianum: the function of the gene mfs1. J Microbiol 51(1):70–81

    Article  CAS  Google Scholar 

  22. Polidori E, Ceccaroli P, Saltarelli R, Guescini M, Menotta M (2007) Hexose uptake in the plant symbiotic ascomycete Tuber borchii Vittadini: biochemical features and expression pattern of the transporter TBHXT1. Fungal Genet Biol 44(3):187–198

    Article  CAS  Google Scholar 

  23. Saitoh H, Hirabuchi A, Fujisawa S, Mitsuoka C, Terauchi R, Takano Y (2014) MoST1 encoding a hexose transporter-like protein is involved in both conidiation and mycelial melanization of Magnaporthe oryzae. FEMS Microbiol Lett 352:104–113

    Article  CAS  Google Scholar 

  24. Scindiya M, Malathi P, Kaverinathan K, Viswanathan R, Ramesh Sundar A (2017) Molecular characterization of pathogenicity gene homologs in Colletotrichum falcatum causing red rot in sugarcane. Sugar Tech 19:563–572

    Article  CAS  Google Scholar 

  25. Scindiya M, Malathi P, Kaverinathan K, Ramesh Sundar A, Viswanathan R (2018) RNA-mediated silencing of PKS1 gene in Colletotrichum falcatum causing red rot in sugarcane. Eur J Plant Pathol 153:371–384

    Article  Google Scholar 

  26. Srinivasan KV, Bhatt NR (1961) Criteria for grading resistance of sugarcane varieties to red rot (Glomeralla tucumanensis). Curr Sci 30(11):425

    Google Scholar 

  27. Tonukari NJ, Scott-Craig JS, Walton JD (2000) The Cochliobolus carbonum SNF1 gene is required for cell wall-degrading enzyme expression and virulence on maize. Plant Cell 12:237–247

    CAS  PubMed  PubMed Central  Google Scholar 

  28. Tzima AK, Paplomatas EJ, Rauyaree P, Ospina-Giraldo MD, Kang S (2011) VdSNF1, the sucrose non-fermenting protein kinase gene of Verticillium dahliae is required for virulence and expression of genes involved in cell wall degradation. MPMI, Mol Plant Microbe Interact 24(1):129–142

    Article  CAS  Google Scholar 

  29. Viswanathan R, Samiyappan R (2000) Red rot disease in sugarcane: challenges and prospects. Madras Agric J 87:549–559

    Google Scholar 

  30. Voegele RT, Struck C, Hahn M, Mendgen K (2001) The role of haustoria in sugar supply during infection of broad bean by the rust fungus Uromyces fabae. Proc Natl Acad Sci U S A 98(14):8133–8138

    Article  CAS  Google Scholar 

  31. Wahl R, Wippel K, Goos S, Kamper J, Sauer N (2010) A novel high-affinity sucrose transporter is required for virulence of the plant pathogen Ustilago maydis. PLoS Biol 8:1–10

    Article  Google Scholar 

  32. Xie X, Wilkinson HH, Correa A, Lewis ZA, Bell-Pedersen D (2004) Transcriptional response to glucose starvation and functional analysis of a glucose transporter of Neurospora crassa. Fungal Genet Biol 41(12):1104–1119

    Article  CAS  Google Scholar 

  33. Yi M, Park JH, Ahn JH, Lee YH (2008) MoSNF1 regulates sporulation and pathogenicity in the rice blast fungus Magnaporthe oryzae. Fungal Genet Biol 45(8):1172–1181

    Article  CAS  Google Scholar 

  34. Zhang T, Sun X, Xu Q, Zhu C, Li Q, Li H (2013) PdSNF1, a sucrose non-fermenting protein kinase gene, is required for Penicillium digitatum conidiation and virulence. Appl Microbiol Biotechnol 97:5433–5445

    Article  CAS  Google Scholar 

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Acknowledgements

The authors are grateful to the Director, ICAR-Sugarcane Breeding Institute for providing facilities and constant encouragement.

Funding

This study was done as part of ICAR-SBI fund.

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Authors and Affiliations

  1. Plant Pathology Section, Crop Protection Division, ICAR-Sugarcane Breeding Institute, Coimbatore, Tamil Nadu, 641007, India

    M. Scindiya, P. Malathi, K. Kaverinathan, A. Ramesh Sundar & R. Viswanathan

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  1. M. Scindiya
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  2. P. Malathi
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  3. K. Kaverinathan
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  4. A. Ramesh Sundar
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  5. R. Viswanathan
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Correspondence to P. Malathi.

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Scindiya, M., Malathi, P., Kaverinathan, K. et al. Knock-down of glucose transporter and sucrose non-fermenting gene in the hemibiotrophic fungus Colletotrichum falcatum causing sugarcane red rot. Mol Biol Rep 48, 2053–2061 (2021). https://doi.org/10.1007/s11033-021-06140-3

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  • DOI: https://doi.org/10.1007/s11033-021-06140-3

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