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Analysis of Multimerin 1 (MMRN1) expression in ovarian cancer

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Abstract

Ovarian cancer, the most lethal gynecological cancer, is the fifth most common cause of cancer-related deaths in women. A cost-effective and non-invasive early screening method for ovarian cancer is required to reduce the high mortality rate. Saliva is a clinically informative unique fluid, which is useful for novel approaches to prognosis, clinical diagnosis, and monitoring for non-invasive detection of disease. Multimerin1 (MMRN1) is a di-sulfide linked homo-polymeric glycoprotein from EMILIN family. Altered expression of MMRN1 has been reported in hepatocellular carcinoma, cervical cancer, and ovarian cancer. But, its role in epithelial ovarian cancer (EOC) is not clear and well documented. In this study, expression of Multimerin 1 was validated in saliva and tissues of EOC patients and age-matched controls by western blotting, ELISA, RT-PCR, and immunohistochemistry. Significant over expression of MMRN1 was observed by western blot and ELISA in saliva samples of EOC patients. The average concentration of MMRN1 in the saliva of healthy controls was 28.7 pg/ml (SE ± 1.76), 42.53 pg/ml (SE ± 4.06) in low grade and 52.91 pg/ml (SE ± 4.24) with p < 0.01 in high-grade EOC. Upregulated cytoplasmic expression of MMRN1 was observed in EOC tissue by immunohistochemistry. Our results suggest that MMRN1 expression is associated with EOC progression and MMRN1 may be potential biomarker candidates for early-stage EOC detection however further experiments are required in a large cohort to establish this proposition. Also, saliva can be explored as a novel medium for ovarian cancer diagnosis.

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Acknowledgments

AS and VK thank Council of Scientific and Industrial Research, Govt. of India and Indian Council of Medical Research (ICMR) New Delhi, India for their fellowships respectively.

Funding

This work was supported by a grant from Indian Council of Medical Research, India (No.5/13/16/2013/NCD-III).

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  1. Abhinav Saini and Kumari Binita Chandra contributed equally to this work.

Authors and Affiliations

  1. Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 110029, India

    Abhinav Saini, Kumari Binita Chandra, Vikrant Kumar & Savita Yadav

  2. Department of Pathology, All India Institute of Medical Sciences, New Delhi, 110029, India

    Sandeep R Mathur

  3. Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, New Delhi, 110029, India

    J B Sharma & Sunesh Kumar

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  1. Abhinav Saini
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Contributions

SY, JBS and SK conceptualized and designed the study. AS, KBC and VK performed the experiments. SY, AS, KBC and VK analyzed the results. The manuscript was drafted by AS, KBC and VK and edited by SY and VK. All authors have read and approved the final manuscript.

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Correspondence to Savita Yadav.

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Ethical approval was obtained from the ethics committee of All India institute of Medical Sciences, New Delhi to carry out this study (IEC/NP-162/2013).

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Saini, A., Chandra, K.B., Kumar, V. et al. Analysis of Multimerin 1 (MMRN1) expression in ovarian cancer. Mol Biol Rep 47, 9459–9468 (2020). https://doi.org/10.1007/s11033-020-06027-9

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