Muscle contraction stimulates a transient change of myogenic factors, partly related to the mode of contractions. Here, we assessed the response of IGF-1Ea, IGF-1Eb, IGF-1Ec, PGC1α-1, PGC1α-4, and myostatin to the eccentric Vs. the concentric contraction in human skeletal muscle. Ten healthy males were performed an acute eccentric and concentric exercise bout (n = 5 per group). For each contraction type, participants performed 12 sets of 10 repetitions knee extension by the dominant leg. Baseline and post-exercise muscle biopsy were taken 4 weeks before and immediately after experimental sessions from Vastus Lateralis muscle. Genes expression was measured by real-time PCR technique. There was a significant increase in PGC1α-1, PGC1α-4, IGF-1Ea and, IGF-1Eb mRNA after concentric contraction (p ≤ 0.05), while the PGC1α-4 and IGF-1Ec significantly increased after eccentric contraction (p ≤ 0.05). It is intriguing to highlight that; no significant differences between groups were evident for changes in any variables following exercise bouts (p ≥ 0.05). Our results found that concentric and eccentric contractions presented different responses in PGC1α-1, IGF-1Ea, IGF-1Eb, and IGF-1Ec mRNA. However, a similar significant increase in mRNA content was observed in PGC1α-4. Further, no apparent differences could be found between the response of genes to eccentric and concentric contraction.
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Glyceraldehyde 3-phosphate dehydrogenase
High-intensity interval training
Insulin-like growth factor-1
Mechano growth factor
Messenger ribonucleic acid
Maximal voluntary isometric actions
- PGC-1 alpha:
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
Real-time polymerase chain reaction
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We would like to thank the subjects and personnel of the University of Tehran and the Faculty of Medical Sciences for their assistance with the clinical portion of this study.
Any institutes did not fund this study.
Conflict of interest
The authors declare no conflict of interest.
All procedures performed in studies involving human participants were by the ethical standards of the ethical committee of Tehran University (Ethic No: IR.UT.SPORT.REC.1397.029) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed consent was obtained from all individual participants included in the study.
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Taghibeikzadehbadr, P., Shirian, S. & Sabouri, M. Effect of different muscle contraction mode on the expression of Myostatin, IGF-1, and PGC-1 alpha family members in human Vastus Lateralis muscle. Mol Biol Rep (2020). https://doi.org/10.1007/s11033-020-06017-x
- Eccentric contraction
- Concentric contraction
- Gene expression
- PGC-1 alpha