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Particle bombardment technology and its applications in plants

Abstract

Particle bombardment, or biolistics, has emerged as an excellent alternative approach for plant genetic transformation which circumvents the limitations of Agrobacterium-mediated genetic transformation. The method has no biological constraints and can transform a wide range of plant species. Besides, it has been the most efficient way to achieve organelle transformation (for both chloroplasts and mitochondria) so far. Along with the recent advances in genome editing technologies, conventional gene delivery tools are now being repurposed to deliver targeted gene editing reagents into the plants. One of the key advantages is that the particle bombardment allows DNA-free gene editing of the genome. It enables the direct delivery of proteins, RNAs, and RNPs into plants. Owing to the versatility and wide-range applicability of the particle bombardment, it will likely remain one of the major genetic transformation methods in the future. This article provides an overview of the current status of particle bombardment technology and its applications in the field of plant research and biotechnology.

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Acknowledgements

Authors thank to Bio-Rad Laboratories for providing the pictures of Biolistic® PDS-1000/He Particle Delivery System and Helios® Gene Gun. Also thankful to Biologist MSc. Asli Hocaoglu-Ozyigit for her technical support.

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IIO had the idea for the article, KYK performed the literature search and drafted the review, KYK and IIO critically revised the work. Both authors read and approved the final manuscript.

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Correspondence to Ibrahim Ilker Ozyigit or Kuaybe Yucebilgili Kurtoglu.

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Ozyigit, I.I., Yucebilgili Kurtoglu, K. Particle bombardment technology and its applications in plants. Mol Biol Rep 47, 9831–9847 (2020). https://doi.org/10.1007/s11033-020-06001-5

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Keywords

  • Biolistic
  • Gene gun
  • Direct gene transfer
  • Transgenic plants
  • Gene delivery
  • Genetic transformation