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Evaluation of silent information regulator T (SIRT) 1 and Forkhead Box O (FOXO) transcription factor 1 and 3a genes in glaucoma

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Abstract

Analysis of the reactive oxygen species (ROS)-detoxifying biomarkers may elucidate the mitochondrial dysfunction in glaucoma pathogenesis. Therefore, we purposed to investigate the effects of ROS-detoxifying molecules including Silent Information Regulator T1 (SIRT1) and Forkhead Box O 1 (FOXO1) and 3a (FOXO3a) transcription factors in patients with glaucoma. Our analyses included 20 eyes from patients with primary open-angle glaucoma (POAG) and 20 eyes from patients with pseudoexfoliation glaucoma (PXG) who were scheduled for trabeculectomy. After extraction of total RNA from trabecular meshwork tissue, we compared the levels of SIRT1, FOXO1and FOXO3a genes in the oxidative pathway with the level of glyceraldehyde-3 phosphate dehydrogenase (GAPDH), the reference gene, using real-time polymerase chain reaction. Relative gene expression was calculated using the threshold cycle (2−ΔΔCT) method. We observed similarly reduced expression levels of SIRT1, FOXO1, and FOXO3a genes versus GAPDH among patient groups (p = 0.40; p = 0.56; p = 0.35, respectively). This is the first study to identify the role of SIRT1 and FOXOs in human TM with glaucoma. Relative expression levels of SIRT1, FOXO1, and FOXO3a genes versus a control gene (GAPDH) were decreased in POAG and PXG groups. Our results show that SIRT1and FOXOs (1-3a) deserve special attention in the pathogenesis of glaucoma.

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Acknowledgements

We thank Assoc. Prof. Yunus Kasım Terzi for assistance of the analysis and critical reading of the manuscript, Aslı Belen Sağlam for assistance in the preparation of the analysis, and Gozde Ozer for statistical analysis of the study.

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Authors and Affiliations

  1. Department of Ophthalmology, Kağızman State Hospital, Kars, Turkey

    Derya Yaman

  2. Department of Ophthalmology, Atatürk Training and Research Hospital, Ankara, Turkey

    Tamer Takmaz & Nilay Yüksel

  3. Department of Medical Genetics, Başkent University Faculty of Medicine, Ankara, Turkey

    Selin Akad Dinçer & Feride İffet Şahin

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  1. Derya Yaman
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  2. Tamer Takmaz
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  3. Nilay Yüksel
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Contributions

DY, TT, NY, Fİ Şahin: Study design, writing manuscript. DY, TT: Collecting data, stastistical analysis. SAD, FİŞ: Genetic analyses of the samples.

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Correspondence to Derya Yaman.

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The ethic Committee: Yildirim Beyazit University, Ethics Committee of Clinical Research. The ethic approval number: 26379996/23. The ethic approval date: 21.02.2018. The guarantor: DY.

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Yaman, D., Takmaz, T., Yüksel, N. et al. Evaluation of silent information regulator T (SIRT) 1 and Forkhead Box O (FOXO) transcription factor 1 and 3a genes in glaucoma. Mol Biol Rep 47, 9337–9344 (2020). https://doi.org/10.1007/s11033-020-05994-3

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