Skip to main content

Advertisement

Springer Nature Link
Log in

Caveolin-1 (CAV-1) up regulation in metabolic syndrome: all roads leading to the same end

  • Short Communication
  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Metabolic syndrome (MS) is a set of clinical conditions such as insulin resistance, hyperglycemia, systemic arterial hypertension (SAH), dyslipidemia, obesity and high abdominal circumference. Some of these clinical characteristics have been associated with caveolin-1, a caveolae structural protein, responsible for insulin activation, storage and degradation of cholesterol, and so on. Herein we assessed CAV-1 mRNA levels in MS patients comparing to healthy controls (HC) and according patients’ clinical features. We included 87 patients in the study, 25 patients with MS, 30 patients with at least one clinical condition (diabetes, SAH, dyslipidemia, obesity and high abdominal circumference), 13 with two clinical conditions and 19 HC. CAV-1 mRNA levels from peripheral blood samples were assessed by Real Time qPCR using specific Taqman probe. The analysis was performed using ∆Cq method and the statistical tests Shapiro–Wilk, One-Way ANOVA and Mann–Whitney. We found CAV-1 increased mRNA levels in patients with MS (1.645 FC, p = 9.794 × 10−20) and even higher in patients with only one or two clinical conditions (2.215 FC, p = 1.215 × 10−32 and 1.716 FC, p = 4.197 × 10−05, respectively). When individual clinical conditions were observed, individuals with high abdominal circumference and obesity present a significantly up regulation when compared to HC (2.956 FC, p = 0.0004 and 3.643 FC, p = 0.002, respectively). This work indicates that CAV-1 gene expression from whole blood samples is associated to MS clinical conditions and may become a potential target for MS treatment and prevention.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  1. Xavier Moore J, Chaudhary N, Akinyemiju T (2017) Metabolic syndrome prevalence by race/ethnicity and sex in the United States, National Health and Nutrition Examination Survey, 1988–2012. Natl Health Nutr Exam Surv Prev Chron Dis 14:1–16. https://doi.org/10.5888/pcd14.160287

    Article  Google Scholar 

  2. Ross SM (2017) Obesity and metabolic syndrome. Holist Nurs Pract 31:348–352. https://doi.org/10.1097/HNP.0000000000000232

    Article  PubMed  Google Scholar 

  3. Castaneda G, Bhuket T, Liu B, Wong RJ (2018) Low serum high density lipoprotein is associated with the greatest risk of metabolic syndrome among U.S. adults. Diabetes Metab Syndr Clin Res Rev 12:5–8. https://doi.org/10.1016/j.dsx.2017.08.002

    Article  Google Scholar 

  4. Moreira GC, Cipullo JP, Ciorlia LAS et al (2014) Prevalence of metabolic syndrome: association with risk factors and cardiovascular complications in an urban population. PLoS ONE 9:e105056. https://doi.org/10.1371/journal.pone.0105056

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Kramer CK, Zinman B, Retnakaran R (2013) Are metabolically healthy overweight and obesity benign conditions? Ann Intern Med 159:758. https://doi.org/10.7326/0003-4819-159-11-201312030-00008

    Article  PubMed  Google Scholar 

  6. Boucher BJ (2012) Is vitamin D status relevant to metabolic syndrome? Dermatoendocrinology 4:212–224. https://doi.org/10.4161/derm.20012

    Article  CAS  Google Scholar 

  7. Vieira EC, Peixoto MRG, Silveira EA (2014) Prevalence and factors associated with metabolic syndrome in elderly users of the Unified Health System. Rev Bras Epidemiol 17:805–817. https://doi.org/10.1590/1809-4503201400040001

    Article  PubMed  Google Scholar 

  8. Pojoga LH, Underwood PC, Goodarzi MO et al (2011) Variants of the caveolin-1 gene: a translational investigation linking insulin resistance and hypertension. J Clin Endocrinol Metab 96:E1288–E1292. https://doi.org/10.1210/jc.2010-2738

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Chuengsamarn S, Garza AE, Krug AW et al (2013) Direct renin inhibition modulates insulin resistance in caveolin-1-deficient mice. Metabolism 62:275–281. https://doi.org/10.1016/j.metabol.2012.07.013

    Article  CAS  PubMed  Google Scholar 

  10. Cao H, Alston L, Ruschman J, Hegele RA (2008) Heterozygous CAV1 frameshift mutations (MIM 601047) in patients with atypical partial lipodystrophy and hypertriglyceridemia. Lipids Health Dis 7:3. https://doi.org/10.1186/1476-511X-7-3

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Thorn H, Stenkula KG, Karlsson M et al (2003) Cell surface orifices of caveolae and localization of caveolin to the necks of caveolae in adipocytes. Mol Biol Cell 14:3967–3976. https://doi.org/10.1091/mbc.e03-01-0050

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Cohen AW, Hnasko R, Schubert W, Lisanti MP (2004) Role of caveolae and caveolins in health and disease. Physiol Rev 84:1341–1379. https://doi.org/10.1152/physrev.00046.2003

    Article  CAS  PubMed  Google Scholar 

  13. Frank PG, Lisanti MP (2004) Caveolin-1 and caveolae in atherosclerosis: differential roles in fatty streak formation and neointimal hyperplasia. Curr Opin Lipidol 15:523–529. https://doi.org/10.1097/00041433-200410000-00005

    Article  CAS  PubMed  Google Scholar 

  14. Gargalovic P, Dory L (2003) Cellular apoptosis is associated with increased caveolin-1 expression in macrophages. J Lipid Res 44:1622–1632. https://doi.org/10.1194/jlr.M300140-JLR200

    Article  CAS  PubMed  Google Scholar 

  15. Swarup S, Goyal A, Grigorova Y, Zeltser R (2020) Metabolic syndrome. StatPearls Publishing LLC, Treasure Island

    Google Scholar 

  16. Sherling DH, Perumareddi P, Hennekens CH (2017) Metabolic syndrome. J Cardiovasc Pharmacol Ther 22:365–367. https://doi.org/10.1177/1074248416686187

    Article  CAS  PubMed  Google Scholar 

  17. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25:402–408. https://doi.org/10.1006/meth.2001.1262

    Article  CAS  PubMed  Google Scholar 

  18. Cohen AW, Razani B, Wang XB et al (2003) Caveolin-1-deficient mice show insulin resistance and defective insulin receptor protein expression in adipose tissue. Am J Physiol Physiol 285:C222–C235. https://doi.org/10.1152/ajpcell.00006.2003

    Article  CAS  Google Scholar 

  19. Razani B, Combs TP, Wang XB et al (2002) Caveolin-1-deficient mice are lean, resistant to diet-induced obesity, and show hypertriglyceridemia with adipocyte abnormalities. J Biol Chem 277:8635–8647. https://doi.org/10.1074/jbc.M110970200

    Article  CAS  PubMed  Google Scholar 

  20. Cohen AW, Razani B, Schubert W et al (2004) Role of caveolin-1 in the modulation of lipolysis and lipid droplet formation. Diabetes 53:1261–1270. https://doi.org/10.2337/diabetes.53.5.1261

    Article  CAS  PubMed  Google Scholar 

  21. Hnasko R, Lisanti MP (2003) The biology of caveolae: lessons from caveolin knockout mice and implications for human disease. Mol Interv 3:445–464. https://doi.org/10.1124/mi.3.8.445

    Article  CAS  PubMed  Google Scholar 

  22. Chen S, Wang X, Wang J et al (2016) Genomic variant in CAV1 increases susceptibility to coronary artery disease and myocardial infarction. Atherosclerosis 246:148–156. https://doi.org/10.1016/j.atherosclerosis.2016.01.008

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Asker S, Taspinar M, Koyun H et al (2017) Caveolin-1 polymorphisms in patients with severe obstructive sleep apnea. Biomarkers 22:77–80. https://doi.org/10.1080/1354750X.2016.1204007

    Article  CAS  PubMed  Google Scholar 

  24. Garcia GJ, Ruiz MS, Gomez DE, Gomez CJ (2017) Frequency of common polymorphisms in Caveolin 1 (CAV1) gene in adults with high serum triglycerides from Colombian Caribbean Coast. Colomb Med 48:167–173. https://doi.org/10.25100/cm.v48i4.2625

    Article  Google Scholar 

  25. Mora-García G, Gómez-Camargo D, Alario Á, Gómez-Alegría C (2018) A common variation in the caveolin 1 gene is associated with high serum triglycerides and metabolic syndrome in an admixed latin American population. Metab Syndr Relat Disord 16:453–463. https://doi.org/10.1089/met.2018.0004

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Aguirre L, Hijona E, Macarulla MT et al (2013) Several statins increase body and liver fat accumulation in a model of metabolic syndrome. J Physiol Pharmacol 64:281–288

    CAS  PubMed  Google Scholar 

  27. Engin A (2017) The definition and prevalence of obesity and metabolic syndrome. Adv Exp Med Biol 960:1–17. https://doi.org/10.1007/978-3-319-48382-5_1

    Article  CAS  PubMed  Google Scholar 

  28. Catalán V, Gómez-Ambrosi J, Rodríguez A et al (2007) Expression of caveolin-1 in human adipose tissue is upregulated in obesity and obesity-associated type 2 diabetes mellitus and related to inflammation. Clin Endocrinol (Oxf) 68:213–219. https://doi.org/10.1111/j.1365-2265.2007.03021.x

    Article  CAS  Google Scholar 

  29. Helena M, Carvalho C, Nigro D et al (2001) Hipertensão arterial : o endotélio e suas múltiplas funções. Rev Bras Hipertens 8:76–88

    Google Scholar 

Download references

Funding

We acknowledge the Foundation of Science and Technology Support of Pernambuco (FACEPE) that support this work under Grant Number APQ 0952-2.02/15.

Author information

Author notes

  1. Gabriela Montenegro de Souza and Maria Eduarda de Albuquerque Borborema equally contributed to this work.

Authors and Affiliations

  1. Laboratório de Genética e Biologia Molecular Humana, Departamento de Genética, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, 1235, Recife, Pernambuco, CEP 50760-901, Brazil

    Gabriela Montenegro de Souza, Maria Eduarda de Albuquerque Borborema, Thays Maria Costa de Lucena, Ariane Fernandes da Silva Santos, Brenda Regina de Lima & Jaqueline de Azevêdo Silva

  2. Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil

    Maria Eduarda de Albuquerque Borborema, Thays Maria Costa de Lucena, Ariane Fernandes da Silva Santos, Brenda Regina de Lima & Jaqueline de Azevêdo Silva

  3. Divisão de Cardiologia, Departamento de Medicina Clínica, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil

    Dinaldo Cavalcanti de Oliveira

Authors
  1. Gabriela Montenegro de Souza
    View author publications

    Search author on:PubMed Google Scholar

  2. Maria Eduarda de Albuquerque Borborema
    View author publications

    Search author on:PubMed Google Scholar

  3. Thays Maria Costa de Lucena
    View author publications

    Search author on:PubMed Google Scholar

  4. Ariane Fernandes da Silva Santos
    View author publications

    Search author on:PubMed Google Scholar

  5. Brenda Regina de Lima
    View author publications

    Search author on:PubMed Google Scholar

  6. Dinaldo Cavalcanti de Oliveira
    View author publications

    Search author on:PubMed Google Scholar

  7. Jaqueline de Azevêdo Silva
    View author publications

    Search author on:PubMed Google Scholar

Contributions

MEAB and JAS conceived and designed the study. GMS, AFSS and BRL performed experimental assays. MEAB analysed the data and TCL performed statistical analysis. MEAB and GMS wrote the manuscript. JAS reviewed and edited the manuscript. DCO provided the patients and performed the angiography. All authors approved the manuscript.

Corresponding author

Correspondence to Jaqueline de Azevêdo Silva.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The procedures performed in the study involving human participants was approved by the institutional and national research committee PLATAFORMA BRASIL, under register number—16441813.1.0000.5208. This study is in accordance with the ethical standards of the Helsinki declaration of 1964.

Informed consent

All individuals involved in the research agreed and signed the informed consent form. Participation in the study was voluntary and had no impact on medical treatment.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

de Souza, G.M., de Albuquerque Borborema, M.E., de Lucena, T.M.C. et al. Caveolin-1 (CAV-1) up regulation in metabolic syndrome: all roads leading to the same end. Mol Biol Rep 47, 9245–9250 (2020). https://doi.org/10.1007/s11033-020-05945-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-020-05945-y

Keywords