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Royal jelly attenuates gastric mucosal injury in a rat ethanol-induced gastric injury model

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Abstract

The aim of the study was to investigate traditionally used Royal Jelly (RJ) for treating an ethanol-induced gastric ulcer model in rats. A total of 32 Wistar albino male rats were divided into 4 groups of 8: group I = Control, group II = Ethanol, group III = RJ + Ethanol, and group IV = Lansoprazole + Ethanol. In groups II, III, and IV, animals were administered 1 ml of absolute ethanol orally after a 24-h fast to induce ulcer formation. The histopathological changes in the gastric mucosa were determined using hematoxylin-eosin (H&E) staining. Immunohistochemically, inducible nitric oxide (iNOS) and nuclear factor kappa beta (Nf-κβ) markings were evaluated in gastric tissue. Cell death in the gastric mucosa was determined by the TUNEL method. Oxidative status markers, superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), and myeloperoxidase (MPO) levels were determined spectrophotometrically. Expression of the interleukin – 1 beta (IL-1β) and tumor necrosis factor-α (TNF-α) genes in gastric tissues was determined by real-time PCR; and TNF-α, IL-10, and IL-1β levels were determined. RJ was found to inhibit iNOS and Nf-κβ activity in the gastric mucosa and prevent epithelial cell apoptosis. In particular, pro-inflammatory cytokines TNF-α and IL-1β levels were significantly decreased in the RJ + Ethanol group compared to the Ethanol group. In addition, a decrease in the MPO level indicated that RJ prevented tissue damage, especially by preventing inflammatory cell infiltration. The study demonstrated a possible gastroprotective effect of RJ in a rat ethanol-induced gastric ulcer model.

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Acknowledgements

This work was supported by the Research Fund of the Tekirdağ Namık Kemal University. Project Number: NKUBAP.02.GA.18.181.

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Authors and Affiliations

  1. Tekirdağ Namık Kemal University, Faculty of Medicine, Department of General Surgery, Tekirdağ, Turkey

    Yasin Duran & Fatin Rüştü Polat

  2. Tekirdağ Namık Kemal University, School of Health, Department of Emergency and Disaster Management, Tekirdağ, Turkey

    İhsan Karaboğa

  3. Tekirdağ Namık Kemal University, Faculty of Medicine, Department of Histology and Embryology, Tekirdağ, Turkey

    Elif Polat & Zeynep Fidanol Erboğa

  4. Çanakkale Onsekiz Mart University, Faculty of Medicine, Department of Physiology, Çanakkale, Turkey

    Mehmet Akif Ovalı

  5. Çanakkale Onsekiz Mart University, Faculty of Medicine, Department of Biophysics, Çanakkale, Turkey

    Rahime Özlem Öztopuz

  6. Tekirdağ Namık Kemal University, Faculty of Medicine, Department of Medical Biochemistry, Tekirdağ, Turkey

    Aliye Çelikkol & Ahsen Yılmaz

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  1. Yasin Duran
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Contributions

YD conceived and designed the research. YD, IK, and RFP conducted the experiments. AY and AC performed the biochemical analysis and analyzed the data. ZFE and EP performed the histological analysis. IK wrote the manuscript. MAO and ROO performed the gene expression analysis. All authors read and approved the manuscript.

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Correspondence to İhsan Karaboğa.

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Duran, Y., Karaboğa, İ., Polat, F.R. et al. Royal jelly attenuates gastric mucosal injury in a rat ethanol-induced gastric injury model. Mol Biol Rep 47, 8867–8879 (2020). https://doi.org/10.1007/s11033-020-05939-w

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