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Sulfated polysaccharide ascophyllan from Padina tetrastromatica enhances healing of burn wounds by ameliorating inflammatory responses and oxidative damage

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Abstract

Sulfated polysaccharide ascophyllan from marine brown algae has been identified to have burn wound healing properties. Thus, we examined the effects of ascophyllan fraction (AF3) on the inflammatory response and oxidative damage in burn wounds. Full-thickness burn wounds in rats were then treated twice per day with topical AF3 ointment (5%), while control groups were treated with 10% povidone-iodine (positive control) and petroleum jelly-based ointment (negative control). The activity of cyclooxygenase-2 and myeloperoxidase and levels of C-reactive protein, nitric oxide, and proinflammatory cytokines (tumor necrosis factor-α, interleukin-6, and interleukin-1β) were observed to have significantly decreased in peripheral blood mononuclear cells, serum, and wound tissue of the group treated with AF3 ointment on day 8 after wounding. The expression of inducible nitric oxide synthase, endothelial nitric oxide synthase, and vascular endothelial growth factor at the mRNA level was determined to be upregulated in the wound tissue of the AF3 ointment-treated group. After treatment with AF3 ointment, the antioxidant enzyme activity and level of reduced glutathione were upregulated, whereas the content of thiobarbituric acid reactive substances decreased. Treatment of burn wounds using 5% AF3 ointment decreases oxidative damage associated with inflammation deceptively via inhibition of inflammatory enzymes, regulation of proinflammatory cytokines, upregulation of angiogenesis, and activity of antioxidant enzymes.

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Acknowledgements

The authors thankfully acknowledge a research grant from the University Grant Commission as Fellowship for Research to Meritorious Students.

Funding

The research is financially supported by the University Grant Commission.

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Authors and Affiliations

  1. Department of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, United Arab Emirates

    Mohsin Sulaiman

  2. Department of Integrative Agriculture, College of Food and Agriculture, United Arab Emirates University, P.O. Box 15551, Al Ain, United Arab Emirates

    Salem Rashed Alyileili

  3. Department of Biochemistry and Nutrition, Central Institute of Fisheries Technology, Matsyapuri, P.O. Box 682029, Cochin, Kerala, India

    Mahadevan Raghavankutty

  4. Department of Biochemistry, University of Kerala, P.O. Box 695581, Thiruvananthapuram, Kerala, India

    Mohsin Sulaiman, Mahadevan Raghavankutty & G. Muraleedhara Kurup

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  1. Mohsin Sulaiman
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  4. G. Muraleedhara Kurup
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Contributions

The experiments were designed by MS and GMK; performed by MS and MR; and analyzed by MS, SRA, and GMK. The manuscript was written by MS and SRA and reviewed and edited by GMK and MR. The final manuscript was read and approved by all authors.

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Correspondence to Mohsin Sulaiman.

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The authors declare no conflict of interest.

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This study has been approved by the Ethical Committee of the University of Kerala in accordance with the established ethics for lab animal usage and maintenance by the Government of India (Sanctioned Number IAEC-KU 09-10/3(1)-BC-GMK).The experiments were conducted according to guidelines of the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Government of India for lab animals use and care.

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Sulaiman, M., Alyileili, S.R., Raghavankutty, M. et al. Sulfated polysaccharide ascophyllan from Padina tetrastromatica enhances healing of burn wounds by ameliorating inflammatory responses and oxidative damage. Mol Biol Rep 47, 8701–8710 (2020). https://doi.org/10.1007/s11033-020-05914-5

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