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DnaJs, the critical drivers of Hsp70s: genome-wide screening, characterization and expression of DnaJ family genes in Sorghum bicolor

Molecular Biology Reports volume 47, pages 7379–7390 (2020)Cite this article

Abstract

The DnaJ/Hsp40s, are important components in the chaperone machine, and play pivotal roles in plant growth, development and stress tolerance. Sorghum, the semi-arid crop, is the drought resilient, model C4 crop. However, no reports of DnaJs have been available. Genome-wide analysis of Sorghum bicolor revealed 113 DnaJ/Hsp40 genes, classified into four groups; 8 genes in SbDnaJ-A class, 10 in SbDnaJ-B, 82 in SbDnaJ-C and 13 in SbDnaJ-D distributed unevenly on all the 10 chromosomes. Chromosomes 1 and 3 were found hot spots with 22 and 20 genes respectively. All genes displayed large number of introns, with an exception of 11 of the SbDnaJ-C which is devoid of introns. Out of 36 paralogous duplications, 7 tandem and 29 segmental duplications were noticed, indicating the major role of segmental duplications in the expansion. Analysis of digital data revealed tissue and stage-specific expressions. Transcriptional profiling of 12 selected genes representing all 4 classes revealed highly significant expression in leaf followed by root tissues. No expression was noticed in stems with an exception of SbDnaJ-C76. The SbDnaJ-A1, D1, and C subgroup genes displayed upregulation in roots, stems and leaves under cold, inferring the involvement of Hsp40s for cellular protection during cold stress. The results demonstrate that C76 and D1 are the candidate genes associated with multiple abiotic stresses. Present research furnishes valuable information about the role of sorghum DnaJs in abiotic stress response and establishes a foundation for understanding the molecular mechanisms associated with plant development and stress tolerance.

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Data availability

The datasets generated during and/or analyzed during the current study are available as the supplementary information.

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Acknowledgements

MN is thankful to the UGC, New Delhi, for providing fellowship. PBK is thankful to the Vignan’s Foundation for Science, Technology and Research, Guntur for providing Emeritus Fellowship.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Affiliations

  1. Department of Genetics, Osmania University, Hyderabad, 500 007, India

    M. Nagaraju, G. Rajasheker & D. Manohar Rao

  2. Advance Center for Computational & Applied Biotechnology, Uttarakhand Council for Biotechnology (UCB), Silk Park, Prem Nagar, Dehradun, 248 007, India

    Anuj Kumar

  3. Department of Biotechnology, Vignan’s Foundation for Science, Technology & Research, Vadlamudi, Guntur, Andhra Pradesh, 522 213, India

    P. B. Kavi Kishor

  4. Biochemistry Division, ICMR-National Institute of Nutrition, Hyderabad, 500 007, India

    M. Nagaraju

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  1. M. Nagaraju
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  2. Anuj Kumar
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Contributions

PBK, and DMR designed the experiments. MN implemented and collected the data. MN, AK, and RG performed experiments. MN, PBK, and DMR analyzed the results and prepared the manuscript. MN, AK, and PBK revised the manuscript. All authors have read and approved the manuscript.

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Correspondence to D. Manohar Rao or P. B. Kavi Kishor.

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Nagaraju, M., Kumar, A., Rajasheker, G. et al. DnaJs, the critical drivers of Hsp70s: genome-wide screening, characterization and expression of DnaJ family genes in Sorghum bicolor. Mol Biol Rep 47, 7379–7390 (2020). https://doi.org/10.1007/s11033-020-05793-w

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  • DOI: https://doi.org/10.1007/s11033-020-05793-w

Keywords

  • SbDnaJ
  • Hydrophilic proteins
  • Segmental duplications
  • qRT-PCR
  • Sorghum