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Periostin, tenascin, osteopontin isoforms in long- and non-long survival patients with pancreatic cancer: a pilot study

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Abstract

Pancreatic adenocarcinoma (PDAC) is the most frequent histological type of malignancy in the pancreas. Extracellular matrix (ECM), plays a critical role during the process of human carcinogenesis and the possible diversity in matricellular proteins composition of ECM may have a significant impact on the clinical course of PDAC. Aim of this paper was to evaluate the expression of three matricellular proteins, including Periostin (POSTN), Tenascin (TNS) and Osteopontin (OPN), in PDAC from long-survival (LS) and non-long survival (NLS) patients. A total of 30 PDAC were analyzed, 15 from patients that survived more than 60 months after surgery (LS) and 15 that died from the disease within 24 (NLS). RNA was extracted and OPN, TNS and POSTN mRNA levels were evaluated by qRT-PCR. LS and NLS samples showed the same type of POSTN and TN isoforms. On the contrary, OPN seems to be preferentially expressed in NLS PDAC. Moreover, OPNb and OPNc isoforms were expressed exclusively in NLS samples. In conclusion, Our data led to hypothesize a possible relationship between the expression of different isoforms of each of these proteins and the clinical outcome of patients with PDAC.

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Acknowledgements

The authors thank Dr. Simonetta Righi (Biblioteca Centralizzata, Policlinico S. Orsola-Malpighi, Università di Bologna, Bologna, Italy) and Dr. Claudia Benini (Surgery Unit Azienda USL-Maggiore Hospital, Bologna, Italy) for their support in the search of scientific bibliography and technical help.

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Nothing to disclose.

Author information

Author notes

  1. Sirio Fiorino, Michela Visani shares co-authorship.

    Annalisa Pession, Dario de Biase shares senior authorship.

Authors and Affiliations

  1. Internal Medicine Unit, Budrio Hospital, Azienda USL Bologna, Bologna, Italy

    Sirio Fiorino

  2. Department of Medicine, Dipartimento di Medicina Specialistica, Diagnostica e Sperimentale)—Molecular Diagnostic Unit, Azienda USL di Bologna, University of Bologna School of Medicine, Bologna, Italy

    Michela Visani, Giorgia Acquaviva, Giovanni Tallini & Antonio De Leo

  3. Surgery Unit, Azienda USL-Maggiore Hospital, Bologna, Italy

    Michele Masetti & Elio Jovine

  4. Anatomic Pathology Unit, Azienda USL-Maggiore Hospital, Bologna, Italy

    Adele Fornelli

  5. Anatomic Pathology Unit, Arcispedale Santa Maria Nuova—IRCCS, Reggio Emilia, Italy

    Moira Ragazzi

  6. Anatomic Pathology Unit, “F. Addarii” Institute of Oncology and Transplantation Pathology, S. Orsola-Malpighi University Hospital, Bologna, Italy

    Francesco Vasuri

  7. Department of Pharmacy and Biotechnology, Dipartimento di Farmacia e Biotecnologie)—Molecular Diagnostic Unit, Azienda USL di Bologna, University of Bologna, Viale Ercolani 4/2, 40139, Bologna, Italy

    Michela Visani, Daniela Grifoni, Chiara Maria Argento, Thais Maloberti, Annalisa Pession & Dario de Biase

  8. Department of General Surgery and Transplantation, St. Orsola-Malpighi University Hospital, Bologna, Italy

    Matteo Ravaioli

  9. Unit of Gastroenterology and Digestive Endoscopy, Forlì-Cesena Hospital, Forlì-Cesena, Italy

    Carlo Fabbri

Authors
  1. Sirio Fiorino
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  2. Michela Visani
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Corresponding authors

Correspondence to Sirio Fiorino or Dario de Biase.

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All information regarding the human material was managed using anonymous numerical codes. All samples were handled in compliance with the Helsinki Declaration (http://www.wma.net/en/30publications/10policies/b3/). The study was approved by the internal review board committee (Azienda USL of Bologna, number 15035).

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Fiorino, S., Visani, M., Masetti, M. et al. Periostin, tenascin, osteopontin isoforms in long- and non-long survival patients with pancreatic cancer: a pilot study. Mol Biol Rep 47, 8235–8241 (2020). https://doi.org/10.1007/s11033-020-05763-2

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