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Oral administration of Flavonifractor plautii attenuates inflammatory responses in obese adipose tissue

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Abstract

Adipose tissue inflammation enhances the symptoms of metabolic syndrome. Flavonifractor plautii, a bacterium present in human feces, has been reported to participate in the metabolism of catechin in the gut. The precise function of F. plautii remains unclear. We assessed the immunoregulatory function of F. plautii both in vitro and in vivo. In vitro, we showed that both viable and heat-killed F. plautii attenuated TNF-α transcript accumulation in lipopolysaccharide-stimulated RAW 264.7 cells. For the in vivo experiment, male C57BL/6 were placed on a high-fat diet (HFD) for 11 weeks. During the final two weeks on the HFD, the animals were administered with F. plautii by once-daily oral gavage. The oral administration of F. plautii attenuated the increase in TNF-α transcription otherwise seen in the epididymal adipose tissue of HFD-fed obese mice (HFD + F. plautii). The composition of the microbial population (at the genus level) in the cecal contents of the HFD + F. plautii mice was altered considerably. In particular, the level of Sphingobium was decreased significantly, and that of Lachnospiraceae was increased significantly, in the HFD + F. plautii group. Obesity is closely associated with the development of inflammation in adipose tissue. F. plautii may be involved in inhibition of TNF-α expression in inflammatory environments. Our results demonstrated that F. plautii may be useful for alleviating the inflammatory responses of adipose tissue.

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Acknowledgements

This work was supported by JSPS KAKENHI Grant Number 17K15268 to TO. We also thank The Research Center for Support of Advanced Science, Shinshu University, for use of their facilities.

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  1. Ayane Mikami and Tasuku Ogita have contributed equally to this work

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  1. Department of Biomolecular Innovation, Institute for Biomedical Sciences, Shinshu University, Nagano, 399-4598, Japan

    Ayane Mikami, Tasuku Ogita, Fu Namai, Suguru Shigemori, Takashi Sato & Takeshi Shimosato

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Correspondence to Takeshi Shimosato.

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All experimental procedures were carried out in accordance with the Regulations for Animal Experimentation of Shinshu University. All experimental procedures were reviewed by the Committee for Animal Experiments of Shinshu University and found to be compliant with national regulations and guidelines, as specified by Law No. 105 and Notification No. 6. The animal protocol was approved by the Committee for Animal Experiments of Shinshu University as Approval No. 300054.

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Mikami, A., Ogita, T., Namai, F. et al. Oral administration of Flavonifractor plautii attenuates inflammatory responses in obese adipose tissue. Mol Biol Rep 47, 6717–6725 (2020). https://doi.org/10.1007/s11033-020-05727-6

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