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Cardioprotective effects of resveratrol following myocardial ischemia and reperfusion

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Abstract

Resveratrol (RSV), a plant origin polyphenol, has shown beneficial cardiovascular effects. In this study, isolated hearts from male Wistar rats were studied using the Langendorff technique. Following 30 min stabilization, the hearts underwent 30 min global ischemia and 120 min reperfusion. The perfusion solution in the test group contained RSV (10 μM). Hemodynamics of the hearts, the markers of myocardial damage including creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), and troponin I were studied during the study. Furthermore, the infarct size and the markers of oxidative stress including catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione peroxidase (GPX) were assayed in the homogenates of the hearts. The release of nitrite from the hearts and the occurrence of ventricular arrhythmias were also monitored throughout the experiment. Resveratrol caused a significant improvement in the restoration of the mechanical performance of the hearts following myocardial ischemia and reperfusion (MIR). Besides, the infarct size, CK-MB, LDH, and troponin I declined in the test group. Besides, the cardiac release of nitrite increased, and the redox status of the heart was improved as indicated by the levels of CAT, SOD, GPX, and MDA. Finally, the treatment caused significant decreases in the occurrences of single and salvo arrhythmias, ventricular tachycardia, and ventricular fibrillation. The current study suggests strong cardioprotective and antiarrhythmic effects for RSV following MIR.

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Acknowledgement

This study was sponsored by Deputy of Research and Technology, Ferdowsi University of Mashhad (Grant No: 3/33319).

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Authors and Affiliations

  1. Department of Physiology, The School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, PO Box: 91775 1793, Iran

    Hamideh Kazemirad & Hamid Reza Kazerani

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  1. Hamideh Kazemirad
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  2. Hamid Reza Kazerani
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Correspondence to Hamid Reza Kazerani.

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The authors report no conflicts of interest.

Research animals

The animals were raised in the Animal Unit of the School of Veterinary Medicine, Ferdowsi University of Mashhad. All experimental procedures complied with the guidelines of the Animal Ethics Committee of the Ferdowsi University of Mashhad (IR.UM.REC.1398.127).

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Kazemirad, H., Kazerani, H.R. Cardioprotective effects of resveratrol following myocardial ischemia and reperfusion. Mol Biol Rep 47, 5843–5850 (2020). https://doi.org/10.1007/s11033-020-05653-7

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