Abstract
MicroRNAs (miRNAs) play important roles in liver pathologies and they are potential biomarkers for diagnosis of liver diseases progression. Changes in miRNA sera expression can be used as non-invasive biomarkers for hepatocellular carcinoma (HCC). The aim of the study was to identify the miRNome profiling of HCC and its diagnostic value in distinguishing HCC from healthy individuals. Expression profiles of miRNAs in serum samples of 20 HCC patients and 10 healthy controls were detected. Whole miRNome profiling was done using next generation sequencing. Receiver operating characteristic (ROC) analysis was performed to assess the diagnostic performance of the deregulated miRNAs for discriminating HCC cases from healthy controls. MiRNA 142 was highly expressed in HCC (P value = 0.023) while miRNAs 191, 22, and 126 were higher in the controls (P value = 0.005, 0.034, 0.010 respectively). We have identified 5 novel miRNAs and they were highly expressed in HCC than controls. Analysis of ROC curve demonstrated that these deregulated miRNAs can be used as a reliable biomarker for detection of HCC with high diagnostic accuracy (AUC = 0.93). We have detected a panel of serum miRNAs that can be used as a reliable noninvasive screening biomarker of HCC. The study recommends further research to shed light on a possible role of the newly discovered novel miRNAs in HCC pathogenesis.
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10 June 2020
The correct spelling of the 7th authors' name is Mona Watany.
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This research was funded by the ministry of higher education, Egypt.
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A Written informed consent was taken from each patient or relatives. Each patient received an explanation of the purpose of the study.
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All human research procedures followed were in accordance with the ethical standards of the committee responsible for human experimentation (institutional and national), and with the Helsinki Declaration of 1975, as revised in 2013. This study was approved by the ethical committee of the Tanta University, Egypt.
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Elhendawy, M., Abdul-Baki, E.A., Abd-Elsalam, S. et al. MicroRNA signature in hepatocellular carcinoma patients: identification of potential markers. Mol Biol Rep 47, 4945–4953 (2020). https://doi.org/10.1007/s11033-020-05521-4
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DOI: https://doi.org/10.1007/s11033-020-05521-4