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Antibacterial effects of a polypeptide-enriched extract of Rana chensinensis via the regulation of energy metabolism

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Abstract

The improper usage of antibiotics is known to cause widespread antibiotic resistance. In this study, the antibacterial effects of a polypeptide-enriched extract from the skin of the amphibian Rana chensinensis (RCP) were evaluated against the Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa, the Gram-positive bacterium Staphylococcus aureus and the fungus Candida albicans. The mechanisms underlying these effects were also studied, and the minimum inhibitory concentration of RCP was determined for each species. Analyses of the levels of adenosine triphosphates (ATPases), including Na+/K+-ATPase and Ca2+-ATPase, and scanning electron microscopy confirmed that RCP damaged the microbial cell walls and membranes. RCP perturbed microbial metabolism and particularly affected the tricarboxylic acid cycle (TCA), suggesting that this agent downregulated the levels of succinate dehydrogenase, malate dehydrogenase and ATPase activity in cells. Furthermore, RCP caused the leakage of genetic material from all four microbial strains. In conclusion, RCP effectively inhibited the growth of Gram-negative and Gram-positive bacteria and a fungal species by disrupting energy metabolic processes.

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Funding

This work was funded by the Special Projects of the Cooperation between Jilin University and Jilin Province (Grant No. SXGJXX2017-1), and Industrial Technology Research and Development Projects from Development and Reform Commission of Jilin Province (Grant No. 2019C050-8).

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Author notes

  1. Ruochen Wang, Siyu Zhai and Yongtao Liang contributed equally to the project.

Authors and Affiliations

  1. School of Life Sciences, Jilin University, Qianjin Street 2699, Changchun, 130012, Jilin Province, People’s Republic of China

    Ruochen Wang, Siyu Zhai, Yongtao Liang, Lirong Teng, Di Wang & Guirong Zhang

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  1. Ruochen Wang
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  2. Siyu Zhai
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  3. Yongtao Liang
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  4. Lirong Teng
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  5. Di Wang
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  6. Guirong Zhang
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Correspondence to Di Wang or Guirong Zhang.

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Wang, R., Zhai, S., Liang, Y. et al. Antibacterial effects of a polypeptide-enriched extract of Rana chensinensis via the regulation of energy metabolism. Mol Biol Rep 47, 4477–4483 (2020). https://doi.org/10.1007/s11033-020-05508-1

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  • DOI: https://doi.org/10.1007/s11033-020-05508-1

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