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Transcriptome analysis of heat stress response genes in potato leaves

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Abstract

Heat stress has a severe impact on potato growth and tuberization process, always resulting in the decrease of tuber yield and quality. Therefore, it is of great significance for potato breeding to illuminate the mechanism of heat stress on potato and explore heat resistant genes. In this study, two cDNA libraries from normal potato leaves (20 °C day/18 °C night) and potato leaves with 3 days of heat treatment (35 °C day/28 °C night) were constructed respectively. Totally, 1420 differentially expressed genes (DEGs) were identified. The expression patterns of 12 randomly selected genes detected using droplet digital PCR agreed with the sequencing data. Gene ontology analysis showed that these DEGs were clustered into 49 different GO types, reflecting the functional diversity of the heat stress response genes. The results of KEGG pathway enrichment showed the potential biological pathways in which the DEGs were involved, indicating that these pathways may be involved in heat tolerance regulation. Most potato heat transcription factors (StHsfs) and heat shock proteins (StHsps) were not expressed efficiently based on expression profile of these DEGs. StHsp26-CP and StHsp70 were markedly increased after 3 days of heat treatment. These data will be useful for further understanding the molecular mechanisms of potato plant tolerance to heat stress and provide a basis for breeding heat-tolerance varieties.

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Acknowledgements

This research was supported by the funds from the Natural Science Foundation of Jiangsu Province (BK20180519) and the Priority Academic Program Development of Jiangsu Higher Education Institutions: Modern Horticultural Science (PAPD).

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Authors and Affiliations

  1. College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China

    Ruimin Tang, Qing Yang & Wenjiao Zhu

  2. Fredericton Research and Development Centre, Agriculture and Agri-Food Canada, Fredericton, NB, E3B 4Z7, Canada

    Ruimin Tang, Suyan Niu, Xiu-Qing Li, Guanshui Chen & Muhammad Haroon

  3. Department of Soil, Water and Climate, University of Minnesota, 1991 Upper Bufford Circle, St. Paul, MN, 55108, USA

    Sanjay K. Gupta

  4. Institute of Bioengineering, Zhengzhou Normal University, Zhengzhou, 450044, China

    Suyan Niu

  5. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Guanshui Chen

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  1. Ruimin Tang
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Contributions

RT, QY, and XL conceived and designed the research. MH, RT and XL performed ddPCR analysis. RT, SN, GC and MH collected the samples. RT, WZ and SG analyzed the data and performed the bioinformatics analysis. RT and SN determined the starch content of potato tubers. SG detected the reducing sugar content in potato tubers. RT and QY wrote the manuscript. XL and SG edited the English language in this manuscript.

Corresponding authors

Correspondence to Xiu-Qing Li or Qing Yang.

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The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

This research is about transcriptomic analysis in plant (potato). Human participants/animals were not involved in this study.

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Not applicable in this study.

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Qing Yang is the first corresponding author and Xiu-Qing Li is the second corresponding author.

Electronic supplementary material

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Supplementary file1 (XLSX 209 kb) The transcriptome sequencing information of the 1420 differentially expressed genes.

11033_2020_5485_MOESM2_ESM.tif

Supplementary file2 (TIF 88 kb) Expression fold changes of 12 selected genes by RNA sequencing and ddPCR. The value showed the expression changes of DEGs under two different methods, that is DEG expression amount under heat stress/its expression amount under normal condition.

11033_2020_5485_MOESM3_ESM.xlsx

Supplementary file3 (XLSX 32 kb) Gene ontology classification analysis of DEGs between non-stressed potato leaves and heat-stressed potato leaves.

Supplementary file4 (XLSX 23 kb) The information of the differentially expressed genes in 110 KEGG pathways.

11033_2020_5485_MOESM5_ESM.tif

Supplementary file5 (TIF 119 kb) The starch content (a) and reducing sugar content (b) in potato tubers with different treatments. CK contrast check potato tubers (20 °C day/18 °C night for 3 months),HS heat stressed potato tubers (35 °C day/28 °C night for 3 months). At least three independent biological experiments were conducted to determine the above indicators. The results were presented as mean ± SE. The method of Student’s t test was used for statistical analysis. Megazyme K_AMYL Amylose kit and DNS (3, 5-dinitrosalicylic acid) calorimetry were used to determine the starch content and the reducing sugar content of potato tubers, respectively. The average starch content in dry matter of potato tubers under normal growing conditions was 67.74%, which was significantly higher than that of potato tubers under heat stress (50.09%) (a). However, The results of reducing sugar content in potato tubers was contrary to the trend of starch content. Under normal growing conditions, the average content of reducing sugar content was 11.25 mg/g tuber (fresh weight), which was significantly lower than that of potato tubers grown under high temperature (the average content of reducing sugar was 32.87 mg/g tuber) (b). In general, the decrease of starch content and the increase of total reducing sugar content in potato tubers after heat treatment indicated that a large amount of starch was hydrolyzed into reducing sugar in response to heat stress.

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Tang, R., Gupta, S.K., Niu, S. et al. Transcriptome analysis of heat stress response genes in potato leaves. Mol Biol Rep 47, 4311–4321 (2020). https://doi.org/10.1007/s11033-020-05485-5

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