Skip to main content

Advertisement

SpringerLink
Log in

Anticancer and antimetastatic activity of Hypomyces chrysospermus, a cosmopolitan parasite in different human cancer cells

  • Original Article
  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

The importance of microbial natural compounds in drug research is increasing every year and they are used to prevent or treat a variety of diseases. Hypomyces chrysospermus is a cosmopolitan parasite of many Boletaceae members. Since not much work has been conducted to date, this study is undertaken to explore the anticancer effect, including the antiproliferative and antimetastatic activity of Hypomyces chrysospermus. The aim of this study is to determine the antiproliferative and antimetastatic activity of Hypomyces chrysospermus ethyl acetate extract, having antioxidant activity, against A549, Caco2, MCF-7 human cancer and CCD-19 Lu and CCD 841 CoN healthy human cell lines. Firstly, cytotoxic activity was determined by the WST-1 assay. After cell proliferations and anti-metastatic effects were investigated by a real-time cell analysis system (RTCA-DP) and IC50 concentrations were calculated for each cell line. In addition, the expression levels of Apaf-1, TNF and NF-kB mRNA in cancer cells were investigated with RealTime-PCR. The ethyl acetate extract of Hypomyces chrysospermus presented anticancer activities including antiproliferative and antimetastatic effects. Hypomyces chrysospermus as a source of biologically active metabolites can be used as an important resource in the development of new anticancer effective agents.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12
Fig. 13

Similar content being viewed by others

References

  1. Demain AL (2014) Importance of microbial natural products and the need to revitalize their discovery. J Ind Microbiol Biotechnol 41:185–201

    Article  CAS  Google Scholar 

  2. Javad SR, Mehdi SR, Bahare S, Marcello I, Amir R, Dima M, Azam SN, Azam A (2018) In vitro and in vivo assessment of free radical scavenging and antioxidant activities of Veronica persica Poir. Cell Mol Biol (Noisy-le-grand) 64(8):57–64

    Article  Google Scholar 

  3. Gargano ML, Van Griensven LJLD, Isikhuemhen OS, Lindequist U, Venturella G, Wasser SP, Zervakis GI (2017) Medicinal mushrooms: valuable biological resources of high exploitation potential. Plant Biosyst 151(3):548–565

    Article  Google Scholar 

  4. Chatterjee S, Biswas G, Basu SK, Acharya K (2011) Antineoplastic effect of mushrooms: a review. Aust J Crop Sci 5(7):904–911

    Google Scholar 

  5. Endo A, Hasumi K, Yamada A, Shimoda R, Takeshima H (1986) The synthesis of compactin (ML-236B) and monacolin K in fungi. J Antibiot 49:1609–1610

    Article  Google Scholar 

  6. Sanguineti E, Cosulich ME, Salis A, Damonte G, Mariotti MG, Zotti MA (2012) Hemolytic peptide from the mycophilic fungus Sepedonium chrysospermum (Bull.) Fr. Appl Microbiol Biotechnol 94:987–994

    Article  CAS  Google Scholar 

  7. Rogerson CT, Samuels GJ (1989) Boleticolous species of Hypomyces. Mycologia 81(3):413–432

    Article  Google Scholar 

  8. Beug MW, Bessette AE, Bessette AR (2014) Ascomycete fungi of North America: a mushroom reference guide. University of Texas Press, Austin, p 486

    Google Scholar 

  9. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325

    Article  CAS  Google Scholar 

  10. White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal rna genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols. A guide to methods and applications. Academic Press, New York, pp 315–322

    Google Scholar 

  11. Yang H, Liu C, Yang D, Zhang H, Xi Z (2009) Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by four typical nanomaterials: the role of particle size, shape and composition. J Appl Toxicol 29:69–78

    Article  Google Scholar 

  12. Engur S, Dikmen M, Öztürk Y (2016) Comparison of antiproliferative and apoptotic effects of a novel proteasome inhibitor MLN2238 with bortezomib on K562 chronic myeloid leukemia cells. Immunopharmacol Immunotoxicol 38:87–97

    Article  CAS  Google Scholar 

  13. Kaya TE, Dikmen M, Öztürk Y (2016) Effects of DNMT and HDAC inhibitors (RG108 and Trichostatin A) on NGFinduced neurite outgrowth and cellular migration. Int J Pharmacol 12:351–360

    Article  Google Scholar 

  14. Bird C, Kirstein S (2009) Real-time, label-free monitoring of cellular invasion and migration with the xCELLigence system. Nat Methods 6:v

    Article  CAS  Google Scholar 

  15. Solly K, Wang X, Xu X, Strulovici B, Zheng W (2004) Application of real-time cell electronic sensing (RT-CES) technology to cell-based assays. Assay Drug Dev Technol 2:363–372

    Article  CAS  Google Scholar 

  16. Urcan E, Haertel U, Styllou M, Hickel R, Scherthan H, Reichla FX (2010) Real-time xCELLigence impedance analysis of the cytotoxicity of dental composite components on human gingival fibroblasts. Dent Mater 26:51–58

    Article  CAS  Google Scholar 

  17. Dikmen M, Cantürk Z, Engür S (2017) Inhibitory effects of secondary metabolites of halotolerant Aspergillus terreus on angiogenesis. Biomed Res 28(8):3613–3618

    CAS  Google Scholar 

  18. Engür S, Dikmen M (2017) The evaluation of the anti-cancer activity of ixazomib on Caco2 colon solid tumor cells, comparison with bortezomib. Acta Clin Belg 72(6):391–398

    Article  Google Scholar 

  19. Limame R, Wouters A, Pauwels B, Fransen E, Peeters M, Lardon F, Wever OD, Pauwels P (2012) Comparative analysis of dynamic cell viability, migration and invasion assessments by novel real-time technology and classic endpoint assays. PLoS ONE 7:e46536

    Article  CAS  Google Scholar 

  20. Dikmen M, Canturk Z, Tilki EK, Engür S (2017) Evaluation of antiangiogenic and antimetastatic Effects of Penicillium chrysogenum Secondary Metabolites. Indian J Pharm Sci 79(1):49–57

    Article  Google Scholar 

  21. Eisenberg MC, Yangjin K, Li R, Ackerman WE, Kniss DA, Friedman A (1998) Mechanistic modelling of the effects of myoferlin on tumor cell invasion. PLoS ONE 108:20078–20083

    Google Scholar 

  22. Dikmen M, Tilki EK, Engür S, Öztürk Y (2017) Neuritogenic activity of epigallocatechin gallate And curcumin combination on rat adrenal pheochromocytoma cells. Fresenius Environ Bull 26(7):4726–4733

    CAS  Google Scholar 

  23. Liekens S, Erik DC, Johan N (2001) Angiogenesis: regulators and clinical applications. Biochem Pharmacol 61(3):253–270

    Article  CAS  Google Scholar 

  24. Abhay PM, Bahare S, Mehdi SR, Raffaele P, Farzad K, Javad SR, Manisha N (2018) Programmed cell death, from a cancer perspective: an overview. Mol Diagn Ther 22:281–295

    Article  Google Scholar 

  25. Güllü İH, Akalın İ (2005) Metastaz biyolojisi. Üroonkoloji Dergisi 4:16–19

    Google Scholar 

  26. Chakraborty P, Thangaraman V, Thangamani S, Ravi D, Abraham J (2016) Bioactivity of Hypomyces chrysospermus Methanol Extract. Res J Pharm Technol 9(2):157–161

    Article  Google Scholar 

  27. Chu WM (2013) Tumor necrosis factor. Cancer Lett 328(2):222–225

    Article  CAS  Google Scholar 

  28. Benedict CA (2019) What is nuclear factor Kappa B (NF-κB) doing in and to the mitochondrion? Front Cell Dev Biol. https://doi.org/10.3389/fcell.2019.00154

    Article  Google Scholar 

  29. Tyrell C, Neil DP, Caroline LW (2016) NFKB1: a suppressor of inflammation, ageing and cancer. FEBS J 283:1812–1822

    Article  Google Scholar 

  30. Şenel S, Kılıçkap S (2010) Anti-tümör Nekrozis Faktör Tedavi ve Kanser. Cumhuriyet Tıp Dergisi 32(1):132–136

    Google Scholar 

Download references

Acknowledgements

This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) under Grant No. 113Z746. The authors extend their thanks to Dr. Bükay Yenice Gürsu for technical contribution of the dendrogram.

Author information

Authors and Affiliations

  1. Faculty of Pharmacy, Department of Pharmacology, Anadolu University, 26210, Eskisehir, Turkey

    Miriş Dikmen

  2. Tavas Vocational School of Health Services, Pamukkale University, Denizli, Turkey

    Selin Engür Öztürk

  3. Faculty of Pharmacy, Department of Pharmaceutical Microbiology, Anadolu University, 26210, Eskisehir, Turkey

    Zerrin Cantürk

  4. Public Health Research and Application Center, Central Immunology Laboratory, Gazi University, Ankara, Turkey

    Göksu Ceylan

  5. Duzen Norwest Laboratories, Kaptanpasa Street, No: 2, Buyukesat District, 06700, Ankara, Turkey

    Ayşe Betül Karaduman

  6. Faculty of Science and Letters, Department of Biology, Eskisehir Osmangazi University, 26480, Eskisehir, Turkey

    Mustafa Yamaç

Authors
  1. Miriş Dikmen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Selin Engür Öztürk
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zerrin Cantürk
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Göksu Ceylan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ayşe Betül Karaduman
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mustafa Yamaç
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Miriş Dikmen.

Ethics declarations

Conflict of interest

There is no conflict of interest in this study.

Ethical approval

This work was carried out on cell lines (A549, Caco2, MCF-7, CCD-19 Lu and CCD 841 CoN), with no requirement for ethical clearance.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 222 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dikmen, M., Öztürk, S.E., Cantürk, Z. et al. Anticancer and antimetastatic activity of Hypomyces chrysospermus, a cosmopolitan parasite in different human cancer cells. Mol Biol Rep 47, 3765–3778 (2020). https://doi.org/10.1007/s11033-020-05468-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-020-05468-6

Keywords

Search

Navigation