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First genomic microsatellite markers developed for Platonia insignis (Clusiaceae), a Brazilian fruit tree

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Abstract

Platonia insignis is a fruit tree native of Brazil with allogamous and asexual reproduction. The production of fruits is mainly obtained by exploitation of natural populations and the impact of genetic structuring on plant production may be evaluated. For this purpose, codominant and multiallelic markers such as microsatellite are the most suitable, but they need to be developed for this species. Thus, the aim of this work was to develop and validate microsatellite markers for P. insignis. We used Roche 454 GS FLX sequencing platform of a single P. insignis genotype and 1702 microsatellite sequences were identified. Based on some pre-requisites, we could develop 50 primer pairs to be tested. Twenty-two primer pairs successfully amplified fragments and they were tested in 31 genotypes of P. insignis that belong to a germplasm bank and were sampled in the northeast of Pará State, Brazil. Thirteen primers were polymorphic and the number of alleles per loci varied from 5 (PI18 and PI27) to 2 (PI08, PI25, PI31, PI33 and PI 37). Expected heterozygosity (HE) varied from 0.74 (PI27) to 0.12 (PI31) and observed heterozygosity (HO) varied from 1.00 (PI25) to 0.00 (PI08, PI31, PI33 and PI37). Principal coordinates could separate the genotypes of P. insignis in clusters and we can conclude that the primers can estimate the genetic diversity of P. insignis populations.

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Acknowledgements

The authors thank Brazilian Agricultural Research Corporation (Embrapa), Process No. 02.11.02.001.00.00 for financial support and the Coordination for the Improvement of Higher Education Personnel (CAPES) for scholarship grants for the first and second authors.

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Authors and Affiliations

  1. Programa de Pós-graduação em Biotecnologia aplicada à Agropecuária, Universidade Federal Rural da Amazônia, Belém, Pará, 66077-530, Brazil

    Luany Caroline Ribeiro Paraense

  2. Programa de Pós-graduação em Agronomia, Universidade Federal Rural da Amazônia, Belém, Pará, 66077-530, Brazil

    Dayane Nascimento Pena

  3. Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, 66077-830, Brazil

    Sylvain Henri Darnet

  4. Laboratório de Genética Molecular, Embrapa Amazônia Oriental, Belém, Pará, 66095-903, Brazil

    Simone de Miranda Rodrigues, Ilmarina Campos de Menezes & Elisa Ferreira Moura

Authors
  1. Luany Caroline Ribeiro Paraense
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  2. Dayane Nascimento Pena
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  3. Sylvain Henri Darnet
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  4. Simone de Miranda Rodrigues
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  5. Ilmarina Campos de Menezes
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  6. Elisa Ferreira Moura
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Correspondence to Elisa Ferreira Moura.

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Paraense, L.C.R., Pena, D.N., Darnet, S.H. et al. First genomic microsatellite markers developed for Platonia insignis (Clusiaceae), a Brazilian fruit tree. Mol Biol Rep 47, 2985–2989 (2020). https://doi.org/10.1007/s11033-020-05301-0

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  • DOI: https://doi.org/10.1007/s11033-020-05301-0

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