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Association of AHSG gene polymorphisms with serum Fetuin-A levels in individuals with cardiovascular calcification in west of Iran

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Abstract

Fetuin-A (AHSG) is a multifunctional secretory protein and acts as an ectopic valve and artery calcification inhibitor. We assessed the correlation between serum levels of Fetuin-A and both exon 6 (248 C/T) and exon 7 (256 C/G) mutations in patients with coronary artery calcification (CAC), mitral annular calcification (MAC), and aortic valve calcification (AVC). 184 patients and 184 healthy individuals as control group were included. The genetic variants of rs4917 and rs4918 for the AHSG gene were determined by PCR-RFLP and T-ARMS PCR techniques. Fetuin-A levels, fasting blood sugar (FBS), urea, creatinine, calcium phosphorus, and lipid profile were measured. Fetuin-A levels were remarkedly lower in individuals with AVC, MAC, and CAC comparing to the control group (p < 0.001). The CT + TT genotypes and the T allele (AHSG Thr248Met) were associated with the risk of calcification of heart valves and coronary artery by 1.31 and 1.27 times in the patient group, respectively. The frequency of CT genotype and T allele was considerably higher in the patient group comparing to the control group. Patients with T allele (CT + TT) had higher levels of FBS, urea, low-density lipoproteins (LDL)-C, phosphorus, systolic blood pressure (SBP), diastolic blood pressure (DBP) while decreased levels of triglyceride, high-density lipoproteins (HDL)-C, calcium and fetuin-A in comparison to control group. Additionally, there was a positive correlation between serum FBS, urea, creatinine, HDL-C, calcium with fetuin-A, and a negative correlation between phosphorous level, SBP, and DBP with fetuin-A. T allele in rs4917 Single nucleotide polymorphism (SNP) is the risk allele of calcification of heart valves and coronary arteries and fetuin-A levels correlates negatively with the occurrence of the disease.

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Abbreviations

AHSG:

α2-Heremance-Schmid –glycoprotein

AVC:

Aortic valve calcification

BMI:

Body mass index

CAC:

Coronary artery calcification

CPPs:

Calciprotein particles

DBP:

Diastolic blood pressure

FBS:

Fasting blood sugar

HDL:

High-density lipoprotein

LDL:

Low-density lipoprotein

MAC:

Mitral annular calcification

PCR:

Polymerase chain reaction

RFLP:

Restriction fragment length polymorphism

SBP:

Systolic blood pressure

SNP:

Single nucleotide polymorphism

TG:

Triglyceride

WT:

Wild type

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Acknowledgements

This work was performed in partial fulfillment of the requirements for the biochemistry MSc thesis of Ehsan M. Noori, in the school of medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran. The authors gratefully acknowledge the Research Council of Kermanshah University of Medical Sciences (Grant Number: 95005) for financial support.

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Authors and Affiliations

  1. Students Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Ehsan Mohammadi-Noori & Saeed Elahirad

  2. Cardiovascular Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Nahid Salehi & Mohammadrza Saidi

  3. Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Hadi Mozafari & Fariborz Bahrehmand

  4. Regenerative Medicine Research Center, Kermanshah University of Medical Sciences, PO-Box: 6714869914, Kermanshah, Iran

    Amir Kiani

  5. Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Asad Vaisi-Raygani

  6. Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Amir Kiani

  7. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Daniel Elieh Ali Komi

  8. Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran

    Daniel Elieh Ali Komi

  9. Department of Internal Medicine Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Ali Moini

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  1. Ehsan Mohammadi-Noori
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  2. Nahid Salehi
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Correspondence to Amir Kiani.

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Mohammadi-Noori, E., Salehi, N., Mozafari, H. et al. Association of AHSG gene polymorphisms with serum Fetuin-A levels in individuals with cardiovascular calcification in west of Iran. Mol Biol Rep 47, 1809–1820 (2020). https://doi.org/10.1007/s11033-020-05275-z

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